Enterocin 55 produced by non rabbit-derived strain Enterococcus faecium EF55 in relation with microbiota and selected parameters in broiler rabbits
Abstract
Ent55 is produced by poultry strain Enterococcus faecium EF55. It is substance which can be allotted to Class II enterocins; thermo-stable, small peptide . Because producer strain has shown beneficial effect in poultry and broiler rabbits as well, we decided to apply Ent55 in broiler rabbit husbandry. Ent55 showed antimicrobial activity in broiler rabbits by reduction of staphylococci, Clostridiae , pseudomonads and coliforms. Its beneficial effect was demonstrated by stimulation of phagocytic activity as well as by reduction of Eimeria spp. oocysts. GPx values were lower; it means, no oxidative stress was evoke d. Moreover, it has not negative influence on growth performance and biochemical parameters. Our results indicated that enterocin produced by not-autochtonous strain can also have protective and beneficial effect in broiler rabbits.
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Introduction
Ent55 is produced by poultry strain Enterococcus faecium EF55 ( 1, 2). It is substance which can be allotted to Class II enterocins; thermo-stable, small peptide. It s maximum inhibition activity achieved after 12 h of cultivation in broth was 12 800 AU/ml. Ent55 has a broad antimicrobial spectrum and its inhibition activity in vivo was demonstrated in Japanese quails ; but its producer strain was shown to have also benefits in chicken . That is , Levkut et al. ( 3) reported inhibition activity of EF55 strain against Salmonella Enteritidis P T4 in chicks. Administration of EF55 strain to ch icks challenged with Salmonella Enteritidis (SE147) resulted in increased number of blood heterophils, caecal IgA+ IEL ( 4). This strain also demonstrated positive impact on intestinal morphometry in the jejunum (5). To have this information we decided to test Ent55 effect in broiler rabbits. The aim was to spread information concerning the Ent55 but also to find beneficial activity in rabbits by this no n rabbit -derived strain producing Ent 55. In last decade, bacteriocins (enterocins) have been tested as biopreservative substances in food industry to prevent contamination by spoilage bacteria ( 6, 7) . However, at recent time , more frequently have been presented studies with application of bacteriocins to protect animals health including our studies (8, 9, 10, 11 ) .
Conclusion
Ent55 showed antimicrobial activity in broiler rabbits by reduction of staphylococci, Clostridiae , pseudomonads and coliforms. Its beneficial effect was demonstrated by stimulation of phagocytic activity as well as by reduction of Eimeria spp. oocysts. GPx values were lower; so no oxidative stress was evoke d. Moreover, it has not negative influence on growth performance and biochemical parameters. Our results indicated that also enterocins produced by not-autochtonous strain can have protective and beneficial effect in broiler rabbits.
