Evaluation of Various Parameters in Mass Multiplication of Beauveria bassiana in Modified Method
Abstract
In the recent years, the environmental contamination caused by excessive use of chemical pesticides increased the interest in integrated pest management, where bio-pesticides are used to control plant pests and plant diseases. Present study deals with use of different media like SDA, rice bran, wheat bran, sorghum and to find their ability as substrates for mass multiplication of beauveria bassiana and creates effective production methodology which can be easily adopted. Biomasses of fungal grain media, organic media and non-synthetic media have been used for the production. Mass multiplication of Beauveria bassiana on different grain media, different temperature like with incubator and without incubator method and calculate biomass of fungus, microscopic examination. Development of SDA was the result which was considered as a best media for quickly growth of Beauveria bassiana and rice bran produced spore production which are most suitable for Beauveria bassiana.
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Introduction
Biopesticide as defined by the United States Environmental Protection Agency, biopesticides are certain types of pesticides derived from such natural materials as animals, plants, bacteria and certain minerals.
In commercial terms, biopesticides include microorganisms that control pests, naturally-occurring substances that control pests, and pesticidal substances produced by plants containing added genetic material. The EPA separates biopesticides into three major classes based on the type of active ingredient used, namely, biochemical, plant incorporated protectants and microbial pesticides. Biochemical pesticides, which are naturally occurring substance that control pest by nontoxic mechanisms. Though biopesticides cover about 1% of the total plant protection products globally, their number and the growth rate have been showing an increasing trend in the past two decades about 175 biopesticides active ingredients and 700 products have been registered worldwide. Regulatory system favorable to chemical pesticide and the gradual disappearance of multiple or mixed cropping, which is known to keep away the magic bullet chemical pesticide. The main advantages of these biocontrol agents are their specificity to target pests, safety to the non-target organism, they do not cause ill effects on environment and human health and can be used against pests which develop resistance to the conventional insecticides, and they fit as ideal components in integrated pest management.
Conclusion
Using biological control agents such as entomopathogenic fungi can be used as a component of integrated pest management of many pests. Several fungal species such as Beauveria bassiana are being used as biocontrol agents for a number of crops, livestock and human nuisance pests. Various agriculture products and by-products such as grain, vegetable waste, seeds, rice husk, and sawdust were evaluated for mass multiplication. Here we take wheat bran, rice bran and sorghum for mass multiplication. Beauveria bassiana effect on rice weevil, Sitophilus oryzae.and rice leaf folder in rice grain.
