Microbia in Plant Growth Promoting Rhizobacteria Bamboo, Reed Grass and Banana
Abstract
Plant Growth Promoting Rhizocbacteria (PGPR) is a type of bacteria that lives around plant roots. These bacteria live in colonies covering the roots of plants so as to provide benefits for plants. The purpose of this study was to determine the types of bacteria found in PGPR bamboo roots, reed roots and banana roots. The research was conducted in the Plant Diseases Pests laboratory, Faculty of Agriculture, Mulawarman University. Isolation of PGPR bacteria was carried out by taking samples from the three PGPR solution materials. Then 2 ml of each PGPR sample was taken and grown on Nutrient Agar (NA) media by the scatter method. From each PGPR made in 4 (four) petri dishes, in order to obtain as many as 12 isolates of PGPR bacteria capable of growing on the media. Some of the genera included in the PGPR are Pseudomonas, Serratia, Azotobacter, Azospirillum, Acetobacter, Burkholderia, Enterobacter, Rhizobium, Erwinia, Flavobacterium and Bacillus. Each rhizobacteria isolate has an important role in controlling pathogen attack and triggering growth. Bacterial analysis is used as a parameter to determine the effectiveness and potential contained in these bacteria.
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Introduction
Plant Growth Promoting Rhizocbacteria (PGPR) is a type of bacteria that lives around plant roots. These bacteria live in colonies covering the roots of plants so as to provide benefits for plants. Some of the genera included in the PGPR are: Pseudomonas, Serratia, Azotobacter, Azospirillum, Acetobacter, Burkholderia, Enterobacter, Rhizobium, Erwinia, Flavobacterium and Bacillus [1]. Each rhizobacteria isolate has an important role in controlling pathogen attack and triggering growth. PGPR bacteria can be inoculated from various plant roots such as bamboo roots, reeds and banana roots. It is necessary to analyze the type and number of bacteria contained in the PGPR isolate used before being applied to cultivated plants.
Based on the results of research that has been carried out, the application of PGPR biological agents that have an effect in delaying the incubation period, it is necessary to analyze the type and number of bacteria contained in the PGPR isolate used before being applied to cultivated plants and suppress the intensity of the attack so that the severity of the disease is not too high. Bacterial analysis is used as a parameter to determine the effectiveness and potential contained in these bacteria. PGPR has properties as a bioprotectant that can protect plants from pathogen attacks [2].
Conclusion
Based on the results of the study, it can be concluded that the results of the identification of bacteria in PGPR of bamboo roots, alang-alang roots and banana roots microscopically showed that the bacteria were Gram Positive and Negative and in the form of bacilli and cocci. The bacteria found were dominated by basillieae bacteria. To obtain perfect identification results, it is necessary to carryout further research with biochemical tests.