Study on Distribution of Microbial and Diazotrophic Azotobacter Population in Five Different Rhizosphere Soil
Abstract
The current scenario encounters the steady demand of food supply and the application of fertilizer is become an indispensable in modern agriculture. Hence, the present study is focused on distribution of microbial and diazotrophic Azotobacter population in rhizosphere soil sample. Total microbial population viz, bacteria, fungi, actiomycetes and diazotrophic Azotobacter were enumerated from five different rhizosphere soil samples and compared the distribution of diazotrophic Azotobacter with other three microbial groups. The results of enumeration of total microbial population reveals that comparatively the bacterial population found to be higher followed by Azotobacter population in various rhizosphere soil. Based on the predominant growth, five Azotobacter strains (one isolate from each sample) were isolated and authenticated through morphological and biochemical characteristics. Keyword sā Diazotrophs, Microbial population, Nitrogen fixation, Azotobacter .
Keywords
Download Options
Introduction
Soil is the blend of minerals, natural matter, gasses, fluids, and the incalculable living beings that together bolster life on Earth. Soil microorganisms advance physicochemical changes in the dirt through the adjustment of soil natural matter, nitrogen obsession and different modifications in soil properties essential for plant growth1.
Nitrogen is extremely plenteous in nature and frequently restrains plant 2 . Microorganisms are essential segments of soil and straightforwardly or in a roundabout way impact the dirt's wellbeing through their useful or deterimental exercises. Rhizosphere microorganisms intercede soil forms through deterioration, supplement assembly and mineralization, nitrogen obsession and denitrification. Most by far of life forms have adjusted to process N by different ecological sources3 .
So as to accomplish the consistent interest of nourishment supply, use of compost is key in present day farming. Consistent utilization of compound manure prompts genuine ecological concerns4 . Accordingly, a requirement for finding an option technique for manageable horticultural practices. Biofertilizer consider to be one such significant option for horticulture practices 5 .
Biofertilizers are comprehensively named nitrogen fixers (N-fixers), potassium solubilizer (K-solubilizers) and phosphorus solubilizers (P-solubilizers)6 . The real biofertilizers being used were Rhizobium, Azotobacter, Azospirillum and phosphate solubilizing bacteria7 . Azotobacter is has a place with the family Azotobacteriaceae and regularly found in the dirt. It is extremely compelling for the change of soil ripeness and harvest efficiency. It can, fix nitrogen specifically from the environment that helps the plants for better grain production8 .Azotobacter application turn into a less expensive, low capital serious other than being eco-accommodating.
In perspective of every one of these focuses, present study is centered around conveyance of microbial and diazotrophic Azotobacter populace in five diverse rhizosphere soil tests.
Conclusion
In this study, five diazotrophic isolates were isolated from rhizosphere soil samples (one from each soil sample) Based on morphological and biochemical characteristics, and all five selected diazotrophic bacterial strains were authenticate as Azotobacter sp. In conclusion, the selected Azotobacter strains have the potential of biological nitrogen fixation and thus it could be used a s biofertilizer for sustainable agriculture practice.
