Study on the Extraction Technology of Ginkgo Biloba Leaf Extract by Enzymolysis Combined with Fermentation
Abstract
In this paper, we select Ginkgo biloba leaves in Taizhou as raw materials and use cellulase and pectinase to hydrolyze Ginkgo biloba leaves, and then the Ginkgo biloba leaves extract was prepared by microbial fermentation. Firstly, cellulase and pectinase were selected for single factor experiment and orthogonal experiment to determine the effect of enzyme dosage, enzymolysis time, temperature and pH value on the extraction rate of Ginkgo biloba leaves; then, microbial fermentation was used to study the effect of optimal temperature, time and pH value on the extraction rate of Ginkgo biloba leaves. The results showed that: the optimal enzyme content was 0.2%, the time of enzymolysis is 2 h, the temperature of enzymolysis was 4 oC, the pH of enzymolysis was 4.5; the optimal microorganism content of fermentation was 4%, the temperature of fermentation was 30oC, the time of fermentation was 8 D, the pH of fermentation was 5，and extraction rate was 18.56%.
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Introduction
Ginkgo biloba is the dry leaf of Ginkgo biloba, a plant of the Ginkgo family, which likes to grow in sunny soil. Ginkgo biloba can promote blood circulation to remove blood stasis, relieve collaterals and relieve pain, and is mostly used for hyperlipidemia. Ginkgo biloba mainly has the effects of improving cardiovascular and cerebrovascular functions, anti-aging, and anti-tumor [1]. At present, the preparation of Ginkgo biloba extract mainly includes chemical methods, microwave, and enzymatic methods [2]. In order to make full use of the effective substances in Ginkgo biloba leaves, enzymatic hydrolysis is used to hydrolyze cellulose and pectin to turn large molecules into small molecules. Fermentation is used to remove toxic substances and the conditions are mild.
Conclusion
In this paper, ginkgo was used as raw material, and the results of enzymatic hydrolysis and fermentation were determined according to the extraction rate through single factor experiment and orthogonal experiment of enzymatic hydrolysis and fermentation. The optimal enzyme addition amount for enzymolysis is 0.2%, the enzymolysis time is 2h, the enzymolysis temperature is 45oC, and the enzymolysis pH is 4.5; the optimal microorganism addition amount for fermentation is 4%, the fermentation temperature is 30oC, the fermentation time is 8d, and the fermentation pH is 5, The extraction rate was 18.56%. ACKNOWLEDGMENT This work is supported by “Taizhou Science and Technology Supporting Plan Social Development Project (TS201923); Taizhou Science and Technology Supporting Plan Agriculture Project (TS201815); General Guidance Project of Jiangsu University Students’ Practice and Innovation in 2019 (201912806012y)”. We are very grateful to Shuping Li,Yanlin Liu,Jinshuang Qi, Xia Li, Fangyue Rong, Jinyong Zhang, Guorui Zhang, Si Chen, Yaxing Guo, Siyu Liu, Chen Liang, Guoai Lufor his technical support and help.