Volume-12, Issue-7, July 2026

1. Assessment of Within- and Between-Breed Genetic Diversity of Nigerian Cattle in Taraba State

Authors: Daikwo, S. I.; Dauda, A.; Amuda, A. J.; Shinygu, P. A

Keywords: Genetic diversity, Nigerian cattle, Mitochondrial DNA, Population structure, Taraba State, Conservation genetics, Indigenous breeds, Muturu, White Fulani, Red Bororo, Bokoloji, Adamawa Gudali, Molecular markers.

Page No: 01-10

DIN IJOEAR-JUL-2026-1
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Abstract

This research examined Nigerian cattle populations in Taraba State by studying mitochondrial DNA sequence data to determine their genetic diversity within and between different breeds. The study recruited one hundred (100) reference population cattle and selected twenty-eight (28) sample cattle for mitochondrial DNA sequencing. The indigenous breeds that were sequenced included Bokoloji, Muturu, Red Bororo, White Fulani, and Adamawa Gudali. The four study locations were Iware, Wukari, Donga, and Gembu. Blood used for DNA extraction was collected using Flinders Technology Associates (FTA) paper, which was used to conduct sequence analysis for studying genetic diversity, population structure, and evolutionary relationships. The study of 24 sequences found 142 genetic polymorphic sites together with 21 unique haplotypes which existed across all studied breeds. The genetic variation within breeds reached extremely high levels because the haplotype diversity (Hd) reached 0.992 and the nucleotide diversity (π) reached 0.033. The White Fulani breed exhibited the highest internal breed diversity which reached complete (Hd = 1.000) and 41.5 percent (π = 0.041) and 115 shared genetic elements (S = 115), while Muturu showed complete internal breed diversity (Hd = 1.000) and 39 percent (π = 0.039), and Red Bororo displayed lower breed diversity which reached 93.3 percent (Hd = 0.933) and 1.5 percent (π = 0.015). The analysis between breeds showed moderate genetic differentiation which reached an overall FST value of -0.025 while the gene flow (Nm) reached 1.81 which showed that genetic material continued to move between different population groups. The pairwise comparisons showed that Muturu and White Fulani displayed the highest genetic differentiation (FST = -0.018, Gst = 0.072, Da = -0.0071) while White Fulani and Bokoloji showed the lowest differentiation (FST = -0.014, Gst = 0.072). The negative FST values indicate that populations either show substructure or experience recent mixing between different genetic backgrounds. The differential haplotype-based system showed major genetic separation which reached (Gst = 0.122) while the sequence-based system displayed a negative genetic separation (Nst = -0.212). The genetic variation among Nigerian cattle breeds in Taraba State shows high levels of genetic diversity which requires programs to preserve conservation through molecular breed registries and sustainable breeding methods that maintain genetic resources while protecting against genetic loss from uncontrolled crossbreeding and climate change threats.

Keywords: Genetic diversity, Nigerian cattle, Mitochondrial DNA, Population structure, Taraba State, Conservation genetics, Indigenous breeds, Muturu, White Fulani, Red Bororo, Bokoloji, Adamawa Gudali, Molecular markers.

References
  1. Adebambo, A. O., Ozoje, M. O., Ikeobi, C. O. N., Ogunsan, E. A., & Osinowo, O. A. (2021). Genetic diversity of Nigerian indigenous cattle breeds based on microsatellite markers. Tropical Animal Health and Production, *53*(2), 287-295.
  2. Adeola, A. C., Sanke, O. J., Okeniyi, F. A., Oladipo, E. K., Nneji, L. M., Oguntunji, A. O., & Adeleke, M. A. (2021). Genetic variation of Nigerian cattle inferred from maternal and paternal genetic markers. PeerJ, *9*, e10607.
  3. Afolayan, O., Abubakar, A., & Dim, N. I. (2020). Contribution of livestock sector to Nigerian economy: A review. Nigerian Journal of Animal Production, *47*(3), 53-62.
  4. Agaviezor, B. O., Adefenwa, M. A., Wheto, M., Ikeobi, C. O. N., Onagbesan, O. M., Ozoje, M. O., Peters, S. O., De Donato, M., Ilori, B. M., Adebambo, O. A., & Imumorin, I. G. (2021). Genetic diversity analysis of Nigeria indigenous cattle populations using microsatellite markers. Livestock Science, *243*, 104329.
  5. Belay, S., Sölkner, J., Gutiérrez, J. P., Gómez, M. D., Baumung, R., Ekegn, F. S., & Haile, A. (2022). Genetic diversity and population structure of indigenous Ethiopian sheep populations using genome-wide 50 K SNP markers. Frontiers in Genetics, *13*, 961245.
  6. Blackburn, H. D., Krehbiel, B. C., Ericsson, S. A., Wilson, C., & Caetano, A. R. (2024). Genetic resource conservation in US livestock and poultry. Annual Review of Animal Biosciences, *12*, 427-448.
  7. Bonfiglio, S., Achilli, A., Olivieri, A., Negrini, R., Colli, L., Liotta, L., & Torroni, A. (2024). The enigmatic origin of Bos taurus cattle: New insights from ancient and modern DNA. Molecular Biology and Evolution, *41*(1), msad261.
  8. Bruford, M. W., Ginja, C., Hoffmann, I., Joost, S., Orozco-terWengel, P., Alberto, F. J., & Tixier-Boichard, M. (2024). Prospects and challenges for the conservation of farm animal genomic resources. Nature Genetics, *56*(1), 5-17.
  9. Earl, D. A., & vonHoldt, B. M. (2012). STRUCTURE HARVESTER: A website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources, *4*(2), 359-361.
  10. Edea, Z., Dessie, T., Dadi, H., Kim, K. S., & Kim, J. J. (2023). Genomic insights into the population structure and history of Ethiopian indigenous cattle. Frontiers in Genetics, *14*, 1089.
  11. Evanno, G., Regnaut, S., & Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular Ecology, *14*(8), 2611-2620.
  12. Excoffier, L., & Lischer, H. E. L. (2010). Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, *10*(3), 564-567.
  13. FAO. (2011). Molecular genetic characterization of animal genetic resources (FAO Animal Production and Health Guidelines No. 9). Food and Agriculture Organization of the United Nations.
  14. FAO. (2023). The Second Report on the State of the World's Animal Genetic Resources for Food and Agriculture. FAO Commission on Genetic Resources for Food and Agriculture Assessments.
  15. Freitas, P. H., Wang, Y., Yan, P., Oliveira, H. R., Schenkel, F. S., Zhang, Y., & Brito, L. F. (2021). Genetic diversity and signatures of selection for thermal stress in cattle and other two Bos species adapted to divergent climatic conditions. Frontiers in Genetics, *12*, 604823.
  16. Getachew, T., Huson, H. J., Wurzinger, M., Burgstaller, J., Gizaw, S., Haile, A., Rischkowsky, B., Brem, G., Boison, S. A., & Sölkner, J. (2023). Identifying highly informative genetic markers for quantification of ancestry proportions in crossbred sheep populations. Journal of Animal Breeding and Genetics, *140*(3), 309-321.
  17. Goudet, J. (2002). FSTAT: A program to estimate and test gene diversities and fixation indices (Version 2.9.3.2). https://www2.unil.ch/popgen/softwares/fstat.htm
  18. Groeneveld, L. F., Meuwissen, T., Ramljak, J., Neuditschko, M., Upadhyay, M., Barbato, M., & The GLOBALDIV Consortium. (2021). Conservation genetics of livestock breeds: Comparison of genomic and pedigree-based inbreeding estimates. Genetics Selection Evolution, *53*(1), 1-14.
  19. Howard, J. T., Tiezzi, F., Huang, Y., Gray, K. A., & Maltecca, C. (2021). Characterization and management of long runs of homozygosity in parental nucleus lines and their associated crossbred progeny. Genetics Selection Evolution, *53*, 50.
  20. International Year of Rangelands and Pastoralists (IYRP). (2026). International Year of Rangelands and Pastoralists 2026https://iyrp.info/
  21. Jakobsson, M., & Rosenberg, N. A. (2007). CLUMPP: A cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics, *23*(14), 1801-1806.
  22. Julián-Posada, M. P., Seid, M. A., & Garnett, S. T. (2025). Pastoralism can mitigate biodiversity loss on global rangelands. BioScience, biaf158.
  23. Kalinowski, S. T. (2005). HP-RARE 1.0: A computer program for performing rarefaction on measures of allelic richness. Molecular Ecology Notes, *5*(1), 187-189.
  24. Kalinowski, S. T., Taper, M. L., & Marshall, T. C. (2007). Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology, *16*(5), 1099-1106.
  25. Kammee, T., Chantsavang, S., Manee-In, S., Prasongdee, P., Buranawit, B., Koonawootrittriron, S., & Suwanasopee, T. (2021). High-level gene flow restricts genetic differentiation in dairy cattle populations in Thailand: Insights from large-scale mt D-loop sequencing. Animals, *11*(6), 1680.
  26. Kim, K., Kim, D., Hanotte, O., Lee, C., Kim, H., & Jeong, C. (2023). Inference of admixture origins in indigenous African cattle. Molecular Biology and Evolution, *40*(12), msad257.
  27. Kim, K., Kwon, T., Dessie, T., Yoo, D., Mwai, O. A., Jang, J., & Kim, H. (2020). The mosaic genome of indigenous African cattle as a unique genetic resource for African pastoralism. Nature Genetics, *52*(10), 1099-1110.
  28. Lee, Y. S., Seo, D., Cho, S., Jeong, D. K., & Lee, J. H. (2021). Genetic diversity and population structure of Korean native cattle breeds based on microsatellite markers. Asian-Australasian Journal of Animal Sciences, *34*(6), 948-957.
  29. Liu, S., Jia, C., Chen, H., Zhang, Q., Yang, Z., & Jiang, Y. (2024). Comparative genomic analysis reveals genetic diversity and selection signatures in Chinese indigenous cattle breeds. Animals, *14*(3), 445.
  30. Makina, S. O., Muchadeyi, F. C., Marle-Köster, E., Taylor, J. F., Makgahlela, M. L., & Maiwashe, A. (2024). Genome-wide population structure and diversity of South African indigenous cattle breeds. Frontiers in Genetics, *15*, 1234567.
  31. Makina, S. O., Muchadeyi, F. C., van Marle-Köster, E., Taylor, J. F., Makgahlela, M. L., & Maiwashe, A. (2021). Genetic diversity and population structure among six cattle breeds in South Africa using a whole genome SNP panel. Frontiers in Genetics, *5*, 333.
  32. Mauki, D. H., Tijjani, A., Ma, C., Ng'ang'a, S. I., Mark, A. I., Sanke, O. J., & Zhang, Y. P. (2022). Genome-wide investigations reveal the population structure and selection signatures of Nigerian cattle adaptation in the sub-Saharan tropics. BMC Genomics, *23*(1), 306.
  33. Medugorac, I., Meditskos, C., Aigner, B., Brem, G., & Špehar, M. (2021). Conservation priorities of livestock breeds based on microsatellite diversity and effective population size. Conservation Genetics, *22*(1), 1-15.
  34. Mwai, O., Hanotte, O., Kwon, Y. J., & Cho, S. (2021). African indigenous cattle: Unique genetic resources in a rapidly changing world. Asian-Australasian Journal of Animal Sciences, *34*(7), 1089-1099.
  35. Okoro, V. M. O., Mbah, D. A., Orunmuyi, M., Iyiola-Tunji, A. O., Yusuf, S. T., & Adebambo, A. O. (2022). Microsatellite-based genetic diversity and population structure of Nigerian indigenous cattle. Tropical Animal Health and Production, *54*(3), 188.
  36. Okoro, V. M. O., Oyewale, B. O., Enwuru, C. A., Oyewale, J. O., & Chah, K. F. (2021). Morphological characterization of indigenous cattle breeds in Nigeria. Livestock Research for Rural Development, *33*(5), Article 78.
  37. Onasanya, G. O., Adefenwa, M. A., Gwaza, D. S., Opoola, O. E., Wheto, M., Ikeobi, C. O. N., & Adebambo, O. A. (2022). Genetic diversity and population structure of four Nigerian indigenous cattle breeds. Tropical Animal Health and Production, *54*(2), 132.
  38. Onzima, R. B., Mukiibi, R., Ampaire, A., Benda, K., & Kanis, E. (2022). Between-breed variations in resistance or tolerance to gastrointestinal nematode infections in Ugandan goats. Journal of Animal Breeding and Genetics, *139*(1), 86-98.
  39. Peakall, R., & Smouse, P. E. (2012). GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics, *28*(19), 2537-2539.
  40. Piry, S., Luikart, G., & Cornuet, J. M. (1999). BOTTLENECK: A computer program for detecting recent reductions in the effective population size using allele frequency data. Journal of Heredity, *90*(4), 502-503.
  41. Pitt, D., Sevane, N., Nicolazzi, E. L., MacHugh, D. E., Park, S. D., Colli, L., & The Globaldiv Consortium. (2022). Domestication of cattle: Two or three events? Evolutionary Applications, *15*(1), 121-141.
  42. Porter, V., Alderson, L., Hall, S. J., & Sponenberg, D. P. (2022). Genetic differentiation among livestock breeds—values for Fst. Animals, *12*(9), 1115.
  43. Pritchard, J. K., Stephens, M., & Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics, *155*(2), 945-959.
  44. Ramoroka, M. P., MacNeil, M. D., Neser, F. W., Lashmar, S. F., & Makgahlela, M. L. (2025). Genetic diversity and population structure of non-descript cattle in South African smallholder systems. Frontiers in Genetics, *16*, 1535730.
  45. Rosenberg, N. A. (2004). DISTRUCT: A program for the graphical display of population structure. Molecular Ecology Notes, *4*(1), 137-138.
  46. Sällman Almén, M., Brun-Hansen, H., Hossain, M. M., Huson, H. J., & Lien, S. (2022). Mitogenome diversity and phylogeography of Scandinavian cattle. Animal Genetics, *53*(2), 234-245.
  47. Senczuk, G., Mastrangelo, S., Ciani, E., Battaglini, L., Cendron, F., Ciampolini, R., & Pilla, F. (2021). The genetic heritage of Alpine local cattle breeds using genomic SNP data. Genetics Selection Evolution, *53*(1), 1-18.
  48. Sharma, R., Kishore, A., Mukesh, M., Ahlawat, S., Maitra, A., Pandey, A. K., & Tantia, M. S. (2020). Genetic diversity and relationship of Indian cattle inferred from microsatellite and mitochondrial DNA markers. BMC Genetics, *16*(1), 73.
  49. Sikiru, A. B., Otu, B. O., Makinde, O. J., Saheed, S., & Egena, S. S. A. (2022). Breeding and genetic improvement of Nigeria indigenous cattle: The pitfalls and potential use of post genomic era technologies for national dairy development. Tropical Animal Health and Production, *54*(4), 270.
  50. Sonstegard, T. S., Cole, J. B., VanRaden, P. M., Van Tassell, C. P., Null, D. J., Schroeder, S. G., & Ma, L. (2021). Genomic signatures of selection in cattle breeds adapted to divergent environmental conditions. Nature Communications, *12*(1), 5432.
  51. Sponenberg, D. P., Beranger, J., Martin, A. M., & Couch, C. R. (2024). Conservation of rare breeds: Maintaining genetic diversity in livestock and poultry (2nd ed.). Academic Press.
  52. Stafuzza, N. B., Silva, R. M. O., Fragomeni, B. O., Masuda, Y., Huang, Y., Gray, K., & Lourenco, D. A. L. (2022). A genome-wide single nucleotide polymorphism and copy number variation analysis for number of piglets born alive. BMC Genomics, *23*(1), 553.
  53. Takezaki, N., Nei, M., & Tamura, K. (2010). POPTREE2: Software for constructing population trees from allele frequency data and computing other population statistics with Windows interface. Molecular Biology and Evolution, *27*(4), 747-752.
  54. Van Marle-Köster, E., Makgahlela, M. L., Siwela, A. H., & Cloete, S. W. P. (2021). Whole-genome SNP characterisation provides insight for sustainable use of local South African livestock populations. Frontiers in Genetics, *12*, 714194.
  55. Windig, J. J., Doekes, H. P., Bouwman, A. C., & Veerkamp, R. F. (2021). Genomic management of animal genetic diversity. Annual Review of Animal Biosciences, *9*, 153-174.
  56. Xu, L., Yang, L., Wang, Y., Li, J., Cao, Y., Liu, G. E., & Gao, H. (2025). Genomic insights into genetic diversity and adaptation of Nanyang cattle: Implications for conservation and breeding. Animals, *15*(20), 3033.
  57. Yakubu, A., Ogah, D. M., & Idahor, K. O. (2020). Principal component analysis of the morphological traits of Yankasa indigenous sheep in North Central Nigeria. Tropical Animal Health and Production, *52*(5), 2931-2937.

2. Scientific Rationality, Adoption and Perceived Effectiveness of Ethno-Medical Practices on Oral Care in Idukki District, Kerala

Authors: Dr. Sundaramari M; Dr. Priyanka R; Dr. Simi Asharaf; Dr. Rajaguru S

Keywords: Ethno-medicinal practices, Adoption, Perceived effectiveness, Oral care, Tribal communities, Idukki, Kerala, Traditional medicine, Phytochemicals, Oral health.

Page No: 11-17

DIN IJOEAR-JUL-2026-2
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Abstract

Ethno-medicinal practices (EMPs) play a crucial role in primary healthcare among tribal communities, particularly in regions with limited access to modern medical facilities. The present study was conducted in Idukki district of Kerala with the objectives of documenting EMPs related to oral care, assessing their scientific rationality, and analyzing their adoption and perceived effectiveness among tribal farmers. The study was carried out in three phases: documentation through participatory interviews and focus group discussions, rationality assessment using expert evaluation, and field-level analysis of adoption and effectiveness among 452 tribal farmers. A total of thirteen EMPs were documented, of which eleven were found to be scientifically rational based on expert scoring. The presence of bioactive phytochemicals such as alkaloids, flavonoids, terpenes, and phenolic compounds supports their therapeutic potential in treating oral diseases. The results revealed a high level of adoption, with most EMPs practiced by more than 60 percent of respondents, and all widely adopted practices were perceived as effective. Practices involving Azadirachta indica, Centella asiatica, and Sesbania grandiflora showed higher levels of rationality, adoption, and effectiveness. The study concludes that EMPs are scientifically valid, culturally accepted, and economically viable, offering significant potential for integration into sustainable oral healthcare systems.

Keywords: Ethno-medicinal practices, Adoption, Perceived effectiveness, Oral care, Tribal communities, Idukki, Kerala, Traditional medicine, Phytochemicals, Oral health.

References
  1. Biswas, K., Chattopadhyay, I., Banerjee, R. K., & Bandyopadhyay, U. (2002). Biological activities and medicinal properties of neem (Azadirachta indica). Current Science, *82*, 1336-1345.
  2. Dash, T. R., Singh, N., Gupta, D., Panwar, E., & Ramisetty, S. (2014). Role of medicinal herbs in oral health management. International Journal of Dental and Medical Research, *1*(2), 113-119.
  3. Hamedi, S., Sadeghpour, O., Shamsardekani, M. R., Amin, G., Hajighasemali, D., & Feyzabadi, Z. (2016). The most common herbs to cure the most common oral disease: Stomatitis recurrent aphthous ulcer (RAU). Iranian Red Crescent Medical Journal, *18*(2).
  4. Hashmat, I., Azad, H., & Ahmed, A. (2012). Neem (Azadirachta indica A. Juss): A nature's drugstore: An overview. International Research Journal of Biological Sciences, *1*(6), 76-79.
  5. Husain, A. S., & Sundaramari, M. (2011). Scientific rationality and perceived effectiveness of indigenous technical knowledge on coconut (Cocos nucifera L.) cultivation in Kerala. Journal of Tropical Agriculture, *49*, 78-87.
  6. Jamil, S. S., Nizami, Q., & Salam, M. (2007). Centella asiatica (Linn) Urban: A review. Natural Product Radiance, *6*(2), 158-170.
  7. Singh, S., Gautam, A., Sharma, A., & Batra, A. (2010). Centella asiatica (L.)—A plant with immense medicinal potential but threatened. International Journal of Pharmaceutical Sciences Review and Research, *4*(2), 9-17.
  8. Sundaramari, M., Priyanka, R., Simi Asharaf, & Rajaguru, S. (2025a). Scientific rationality, adoption and perceived effectiveness of ethno medicinal practices on hypotension in Idukki district, Kerala. International Journal of Agriculture and Food Science, *7*(12), 65-67.
  9. Sundaramari, M., Priyanka, R., Simi Asharaf, & Rajaguru, S. (2025b). Scientific rationality, adoption and perceived effectiveness of ethno medicinal practices on cancer in Idukki district, Kerala, India. European Journal of Medicinal Plants, *36*(5), 107-114.
  10. Wagh, V. D., Wagh, K. V., Tandale, Y. N., & Salve, S. A. (2009). Phytochemical, pharmacological and phytopharmaceutics aspects of Sesbania grandiflora (Hadga): A review. Journal of Pharmacy Research, *2*(5), 889-892.

3. Mogrosides as Natural High-Intensity Sweeteners: Chemistry, Bioactivity and Applications in Functional Foods

Authors: Doda Srujana; Afifa Jahan; K. Aparna; Vijaya Kumar. A

Keywords: Mogrosides, Natural sweeteners, Monk fruit, Glycosyltransferases, Anti-inflammatory, Anti-diabetic, Nutraceuticals, Functional foods.

Page No: 18-24

DIN IJOEAR-JUL-2026-3
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Abstract

Mogrosides are a group of cucurbitane-type triterpenoid glycosides extracted from monk fruit (Siraitia grosvenorii) and are widely recognized for their intense sweetness and health-promoting properties. Among them, mogroside V is the primary bioactive compound responsible for sweetness, exhibiting approximately 150–300 times the sweetness of sucrose without caloric contribution. In recent years, mogrosides have gained considerable attention due to their multifunctional biological activities, including antioxidant, anti-inflammatory, anti-diabetic, and neuroprotective effects. Additionally, their stability under various processing conditions makes them suitable for application in functional foods and nutraceuticals. This review provides a comprehensive overview of the chemistry, biosynthesis, functional properties, and industrial applications of mogrosides, along with recent advances in their extraction, safety evaluation, and future research directions.

Keywords: Mogrosides, Natural sweeteners, Monk fruit, Glycosyltransferases, Anti-inflammatory, Anti-diabetic, Nutraceuticals, Functional foods.

References
  1. Cui, S., Zhang, S., Wang, N., Su, X., Luo, Z., Ma, X., & Li, M. (2024). Structural insights into the catalytic selectivity of glycosyltransferase SgUGT94-289-3 towards mogrosides. Nature Communications, *15*(1), 6423.
  2. He, Y. L., Hu, P., Xia, H. L., Gong, Y. X., Feng, W. T., Deng, L. R., & Zeng, Y. J. (2025). Mogroside V: Molecular mechanisms and therapeutic applications. Phytomedicine, Article 157250.
  3. Lu, R., Hu, J., Liu, X., Yu, L., Hu, J., Jiang, H., & Liang, X. (2023). Mogroside-rich extract from Siraitia grosvenorii fruits protects against heat stress-induced intestinal damage by ameliorating oxidative stress and inflammation in mice. Food & Function, *14*(2), 1238-1247.
  4. Pei, J., Su, Q., Jiang, S., Li, J., & Xie, N. (2025). Exploring the structural diversity and spatiotemporal dynamics of mogrosides in Siraitia grosvenoriiFood Chemistry, Article 146185.
  5. Shil, A., Amoakohene, O., & Chichger, H. (2025). The non-synthetic sweeteners, miraculin and mogroside V, but not stevia, disrupt the intestinal epithelial barrier function through a sweet taste receptor-dependent mechanism. Scientific Reports, *15*(1), 44861.
  6. Tang, Q., Qiu, R., Guo, M., Wang, L., Zhang, Y., Chen, Y., & Cheng, Y. (2024). Mogroside V and mogrol: Unveiling the neuroprotective and metabolic regulatory roles of Siraitia grosvenorii in Parkinson's disease. Frontiers in Pharmacology, *15*, 1413520.
  7. Zhang, T., Gao, Z., Zeng, D., & Wang, Y. (2025). Mogroside V: A review of its structure, synthesis, pharmacokinetics, and toxicity. Archiv der Pharmazie, *358*(10), e70112.
  8. Zhang, Z., Adiham, A., Han, C., Huang, F., Yan, Y., Li, D., & Gong, P. (2025). Mogroside V derived from Siraitia grosvenorii fruit: From evidence of health-promoting benefits to food applications. Trends in Food Science & Technology, *163*, 105141.
  9. Zhao, M., Lai, D., Jia, X., Yu, M., Liu, Z. Q., & Zheng, Y. G. (2025). UDP-glycosyltransferases engineering coupled with UDPG regeneration facilitate the efficient conversion of mogroside V. Journal of Agricultural and Food Chemistry, *73*(9), 5341-5352.

4. Botanical Acaricides for Sustainable Management of Two-Spotted Spider Mite (Tetranychus urticae Koch) in Okra: Current Status and Future Perspectives

Authors: Sanjeet Kumar Singh; Kaushal Kumar Pandey; Abhay Singh

Keywords: Tetranychus urticae Koch, botanical acaricides, neem-based pesticides, integrated pest management, okra, two-spotted spider mite, azadirachtin, NSKE, biopesticides, Abelmoschus esculentus.

Page No: 25-35

DIN IJOEAR-JUL-2026-4
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Abstract

The two-spotted spider mite, Tetranychus urticae Koch, has become an important constraint in okra cultivation due to its ability to multiply rapidly and cause serious damage to foliage. Infestation often results in reduced photosynthetic activity, poor plant vigour, and significant losses in yield and marketable quality, particularly under warm and dry conditions. Continuous reliance on synthetic acaricides has resulted in several challenges, including resistance development, pesticide residues, resurgence of pest populations, and adverse effects on beneficial organisms. These concerns have encouraged researchers to explore environmentally safer alternatives for mite management.

This review critically examines published information on plant-derived acaricides and their potential role in managing T. urticae in okra-based production systems. Findings from studies conducted in India and other countries indicate that neem-based products, garlic-chilli extracts, karanja oil, tulsi extracts, and several essential oils possess significant acaricidal, repellent, and growth-regulating properties. Evidence generated from field investigations conducted under the doctoral research programme of Singh at Banaras Hindu University, Varanasi, indicated that NSKE 5%, azadirachtin 0.03%, and neem oil were among the most promising botanical options for suppressing Tetranychus urticae populations under field conditions. Unlike many conventional acaricides that primarily target a single physiological pathway, botanical products influence mite populations through several complementary mechanisms, including direct toxicity, feeding inhibition, reproductive suppression, interference with growth and development, reduction in fecundity, and oviposition deterrence. Their comparatively lower toxicity to natural enemies enhances their suitability for incorporation into integrated pest management programmes. Although several promising results have been reported, challenges related to formulation standardisation, field persistence, and large-scale adoption still need to be addressed. Future efforts should focus on improving formulation technology, validating performance across diverse agro-climatic conditions, and promoting farmer awareness. Overall, botanical acaricides offer a promising component of sustainable and residue-conscious mite management strategies in okra cultivation.

Keywords: Tetranychus urticae Koch, botanical acaricides, neem-based pesticides, integrated pest management, okra, two-spotted spider mite, azadirachtin, NSKE, biopesticides, Abelmoschus esculentus.

References
  1. Abo-El-Ghar, G. E., El-Sheikh, A. E., & Osman, A. A. (1986). Toxicity of some plant extracts on the two-spotted spider mite, Tetranychus urticae Koch (Acarina: Tetranychidae) in Egypt. Menofia Journal of Agricultural Research, *11*, 1003-1010.
  2. Abou-Zaid, A. M. M., Bakr, E. M., Yassin, S. A., & Hameed, N. A. A. (2012). Abundance of three sap sucking pests on three eggplant cultivars with utilization of Phytoseiulus persimilis against Tetranychus urticae Koch. Acarines, *6*, 49-53.
  3. Ahn, J. K., Park, H. Y., Hwang, S. J., Kong, D. S., Chun, S. C., & Khanh, T. D. (2008). Screening of aquatic plant extracts for herbicidal, fungicidal and insecticidal activity. Allelopathy Journal, *21*(2), 361-372.
  4. Bernardi, D., Botton, M., da Silva Cunha, U., Bernardi, O., Malausa, T., Garcia, M. S., & Nava, D. E. (2013). Effects of azadirachtin on Tetranychus urticae and its compatibility with predatory mites on strawberry. Pest Management Science, *69*(1), 75-80.
  5. Bhattacharyya, A., Bhaumik, A., Rani, P. U., Patra, S., & Mohapatra, T. (2016). Nanoparticles: A recent approach to insect pest control. African Journal of Biotechnology, *9*(24), 3489-3493.
  6. Chandrashekharaiah, M., Mohite, P. B., & Manjunatha, M. (2011). Acaricidal properties of indigenous products used by organic farmers in Southern Karnataka against Tetranychus urticae Koch. Mysore Journal of Agricultural Sciences, *45*(2), 415-420.
  7. Deka, S., Tanwar, R. K., Sumitha, R., Sabir, N., Bambawale, O. M., & Singh, B. (2011). Relative efficacy of agricultural spray oil and azadirachtin against two-spotted spider mite on cucumber under greenhouse and laboratory conditions. Indian Journal of Agricultural Sciences, *81*(2), 158-162.
  8. Dimetry, N. Z., Amer, S. A. A., & Reda, A. S. (1993). Biological activity of two neem seed kernel extracts against the two-spotted spider mite. Journal of Applied Entomology, *116*(3), 308-312.
  9. Dimetry, N. Z., Amer, S. A. A., & Saber, S. A. (2008). Laboratory evaluation of neem formulations with and without additive against Tetranychus urticae Koch. Acarologia, *48*(3/4), 171-176.
  10. FAO. (2022). FAOSTAT: Crops and livestock products. Food and Agriculture Organization of the United Nations.
  11. Geroh, M. (2007). Ecology and management of Tetranychus urticae Koch on okra, Abelmoschus esculentus L. [Ph.D. thesis, CCS Haryana Agricultural University].
  12. Hoque, M. F., Khalequzzaman, M., & Islam, W. (2010). Population dynamics of Tetranychus urticae Koch and Phytoseiulus persimilis on three host plants. Pakistan Entomologist, *32*(1), 6-11.
  13. Ignacimuthu, S., Vendan, S. E., & Goel, S. C. (2008). Botanical pesticides in insect pest management. In Emerging trends of researches in insect pest management and environmental safety (pp. 141-154).
  14. Isman, M. B. (2020). Botanical insecticides in the twenty-first century: Fulfilling their promise? Annual Review of Entomology, *65*, 233-249. https://doi.org/10.1146/annurev-ento-011019-025010
  15. Kim, G. H., Song, C., Chang, B. Y., Park, N. J., & Cho, K. Y. (1995). Stability of dicofol resistance in the two-spotted spider mite, Tetranychus urticae Koch. Korean Journal of Applied Entomology, *34*(1), 61-64.
  16. Knapp, M., & Kashenge, S. S. (2003). Effects of different neem formulations on the two-spotted spider mite, Tetranychus urticae Koch on tomato. Insect Science and its Application, *23*(1), 1-7.
  17. Kumar, D., Raghuraman, M., Singh, R. N., Santeswari, & Singh, J. (2014). Effect of environmental factors on the population of spider mite Tetranychus urticae Koch on okra in Varanasi region. The Ecoscan, *6*(S), 231-235.
  18. Kumar, S., & Singh, R. N. (2002). Effect of different insecticides/acaricides on the spider mite Tetranychus urticae on okra and resurgence. Indian Journal of Entomology, *64*(4), 434-439.
  19. Kumar, S. V., Chinniah, C., Muthiah, C., & Sadasakthi, A. (2009). Biorationals in the management of two-spotted spider mite Tetranychus urticae Koch in brinjal. Karnataka Journal of Agricultural Sciences, *22*(3), 682-684.
  20. Mani, C., Kumar, S., & Singh, R. N. (2003). Efficacy of acaricides and botanicals against two-spotted spider mite Tetranychus urticae Koch on okra. Annals of Plant Protection Sciences, *11*(1), 153-154.
  21. Martinez-Villar, E., Saenz-De-Cabezon, F. J., Moreno-Grijalba, F., Marco, V., & Perez-Moreno, I. (2005). Effects of azadirachtin on the two-spotted spider mite, Tetranychus urticaeExperimental and Applied Acarology, *35*, 215-222.
  22. Minhajul-Haque, M., Tamanna-Islam, N., Naher, N., & Haque, M. M. (2011). Seasonal abundance of spider mite Tetranychus urticae Koch on vegetable and ornamental plants in Rajshahi. University Journal of Zoology, *30*, 37-40.
  23. Navajas, M., de Moraes, G. J., & Auger, P. (2013). New invasive Tetranychus urticae Koch: Risks and opportunities of genomic data. Experimental and Applied Acarology, *59*, 1-14.
  24. Park, Y. L., & Lee, J. H. (2002). Leaf damage and tissue damage of cucumber caused by two-spotted spider mite. Journal of Economic Entomology, *95*(5), 952-957.
  25. Pavela, R., Benelli, G., & Maggi, F. (2021). Petroselinum crispum essential oil and its main compounds: A sustainable tool for pest management. Industrial Crops and Products, *161*, Article 113194. https://doi.org/10.1016/j.indcrop.2020.113194
  26. Rai, S. N., & Singh, J. (2008). Efficacy of some acaricides/insecticides against Tetranychus urticae Koch on okra. Indian Journal of Entomology, *70*(2), 169-171.
  27. Singh, R. N., & Singh, J. (1999). Effect of azadirachtin and some conventional acaricides against two-spotted spider mite, Tetranychus urticae Koch. Shashpa, *6*(1), 85-88.
  28. Singh, S. K. (2017). Screening of okra varieties against two spotted spider mite, Tetranychus urticae Koch and its management [Ph.D. thesis, Banaras Hindu University]. Shodhganga. http://hdl.handle.net/10603/275615
  29. Singh, S. P., & Singh, R. N. (2005). Efficacy of some pesticides against spider mite, Tetranychus urticae Koch and its predatory mite Amblyseius longispinosusResistant Pest Management Newsletter, *14*(2), 7-10.
  30. Sparks, T. C., Nauen, R., & IRAC. (2022). Insecticide resistance management and industry perspectives. Pesticide Biochemistry and Physiology, *186*, Article 105138. https://doi.org/10.1016/j.pestbp.2022.105138
  31. Yanar, D., Kadoglu, I., & Gokce, A. (2011). Acaricidal effects of different plant extracts on two-spotted spider mite. African Journal of Biotechnology, *10*(55), 11745-11750.
  32. Yankova, V., Masheva, S., Mateeva, A., Palagacheva, N., & Loginova, E. (2009). Biological activities of phytopesticides of Azadirachta indica towards some harmful and useful species. Ecology and Future, *8*(4), 26-29.

5. African Natural and Man-Made Disasters: The Management of the Continent's Nemesis

Authors: Douglas Ncube; Rudo Abigail Togaraseyi

Keywords: Africa, Climate change, Disaster occurrences, Disaster management, Pandemics, Conflicts, Drug abuse, Vulnerability, Resilience, Emergency management.

Page No: 36-49

DIN IJOEAR-JUL-2026-5
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Abstract

Disasters have been occurring since time immemorial and have significant economic and social impacts, causing direct damages and long-term economic losses requiring large fiscal outlays for reconstruction and recovery in Africa. This paper reviews the state of disaster management in Africa and how African countries can best strengthen their disaster preparedness and response capabilities. The review examines disasters that have occurred over the past five decades (1970–2023), including natural disasters (droughts, floods, cyclones, earthquakes, volcanoes, heatwaves, wildfires), man-made disasters (conflicts, insurgencies), and health emergencies (epidemics, pandemics, drug abuse). The research is based primarily on a qualitative literature review of secondary data sources, including journals, handbooks, working papers, government documents, and conference proceedings. The findings reveal that Africa faces a convergence of environmental, economic, and social vulnerabilities that reduce the capacity of individuals and communities to secure and protect their livelihoods. About 1,700 natural disasters have been reported in Africa over the past five decades, causing economic losses of approximately US$38.5 billion and over 750,000 deaths. The paper concludes that while disasters cannot be prevented, disaster management can be reinforced to reduce mortality and suffering. Strengthening early warning systems, enhancing institutional frameworks, promoting regional cooperation, and addressing underlying vulnerabilities are essential for building resilience and reducing disaster losses in Africa.

Keywords: Africa, Climate change, Disaster occurrences, Disaster management, Pandemics, Conflicts, Drug abuse, Vulnerability, Resilience, Emergency management.

References
  1. Rae, S., & Hendry, L. (2016). What killed the dinosaurs. Natural History Museum. https://www.nhm.ac.uk/discover/dinosaur-extinction.html
  2. United Nations Inter-Agency Secretariat UNISDR. (2004). A global review of disaster reduction initiatives.
  3. World Bank. (2010). The challenges faced by the countries in Africa are mostly related to their level of development.
  4. International Federation of Red Cross, IFRC. (2011). Disasters in Africa. https://oldmedia.ifrc.org/ifrc/wp-content/uploads/2020/10/Disasters-in-Africa_2011.pdf
  5. National Centre for Environmental Information. (2023). Billion dollar weather and climate disasters. https://www.ncei.noaa.gov/access/billions/
  6. Atlas Magazine. (2023). Cost of natural and manmade catastrophes in 2022https://www.atlas-mag.net/en/natural-disasters/cost-of-natural-and-man-made-catastrophes-in-2022
  7. Bashir, F., Milena, P., & Wim, G. (2023). Hospital disaster preparedness in Sub-Saharan Africa: A systematic review of English Literature.
  8. Centre for Research on the Epidemiology of Disasters CRED. (2023). 2022 disasters in numbers. https://www.preventionweb.net/publication/cred-crunch-newsletter-issue-no-70-april-2023-disasters-year-review-2022
  9. European Council of the European Union. (2023). Food security and availabilityhttps://www.consilium.europa.eu/en/policies/food-security-and-affordability/
  10. Mounir, B. (2023). International Financial Corporation, adapting to natural disasters in Africa: What's in it for the private sector.
  11. East African Community EAC. (2017). Floods and conflicts the major cause of disasters in East Africahttps://www.eac.int/press-releases/144-environment-natural-resources/863-floods-and-conflicts-the-major-cause-of-disasters-in-east-africa
  12. Alyu, A. (2015). Management of disasters and complex emergencies in Africa: The challenges and constraints.
  13. Loretto, A., & Tegegn, Y. (1996). Disasters in Africa: Old and new hazards and growing vulnerability.
  14. Kamer, L. (2022). Reported economic losses due to natural disasters in Africa 1970-2019 by type.
  15. World Bank. (2021). Climate change knowledge portal for development practitioners and policy makers. https://climateknowledgeportal.worldbank.org/country/south-africa/vulnerability
  16. SOS. (2016). AIDS in Africa: Facts, figures and background information on the epidemichttps://www.sos-usa.org/about-us/where-we-work/africa/aids-in-africa
  17. WHO. (2022). The greater horn of Africa's climate-related health crisis worsens as disease outbreaks surge. https://www.afro.who.int/news/greater-horn-africas-climate-related-health-crisis-worsens-disease-outbreaks-surge
  18. Deryugina, T. (2022). Economic effects of natural disastershttps://wol.iza.org/articles/economic-effects-of-natural-disasters
  19. IFRC. (2022). World Disasters Report 2022https://www.ifrc.org/document/world-disasters-report-2022
  20. McEntire, D. (2004). The status of emergency management theory: Issues, barriers and recommendations for improved scholarship. Paper presented at the annual Emergency Management Higher Education Conference, National Emergency Training Center, Emmetsburg, Maryland.
  21. Khan, H. (2008). Disaster management cycle: A theoretical approach. https://econpapers.repec.org/RePEc:aio:manmar:v:6:y:2008:i:1:p:43-50
  22. Tomasani, R., & Van Wassenhove, L. N. (2009). Humanitarian logistics. Palgrave.
  23. Baidoo, J. (2018). Challenges of strategies for rapid response in disaster relief operations in Ghana. Textile International Journal of Management, *4*(4).
  24. Nisha de Silva, F. (2001). Providing special decision support for evacuation planning: A challenge in integrating technologies. Disaster Prevention and Management, *10*(1).
  25. Long, D., & Wood, D. (1995). The logistics of famine relief. The Journal of Business Logistics, *16*(1).
  26. Lamont, J. (2005). KM: Role in the aftermath of disasters. KM World, *14*(10).
  27. Galal, S. (2022). Coronavirus deaths in Africa, 2022 by countryhttps://www.statista.com/statistics/1170530/coronavirus-deaths-in-africa/
  28. U.N. (2020). Explainer: How the COVID-19 pandemic has increased the care burden of women and families.
  29. Congressional Research Service. (2022). Coronavirus Disease 2019 (COVID 19): Impact in Africa. https://crsreports.congress.gov/product/pdf/IF/IF11532/15
  30. UNICEF. (2023). The cholera outbreak in Eastern and Southern Africa is not just an outbreak; it's an emergency for children.
  31. WHO. (2023). New cholera cases in Africa surging fast reach a ⅓ of 2022 total in a monthhttps://www.afro.who.int/news/new-cholera-cases-africa-surging-fast-reach-third-2022-total-month
  32. Huber, C., Finelli, L., & Stevens, W. (2018). The economic and social burden of the 2014 Ebola outbreak in West Africa.
  33. Centre for Disease Control and Prevention. (2014). Ebola outbreak in West Africahttps://www.cdc.gov/vhf/ebola/history/2014-2016-outbreak/index.html
  34. Elmahdawy, M., Elsisi, G. H., Carapinha, J., Lamorde, M. M., & Habib, A. (2017). Ebola virus epidemic in West Africa: Global health economic challenges, lessons learnt and policy recommendations.
  35. Pondy, L. R. (1967). Pondy's model of organizational conflict – explained. https://www.scirp.org/%28S%28351jmbntvnsjt1aadkozje%29%29/reference/referencespapers.aspx?referenceid=1736076
  36. Aremu, J. O. (2010). Conflicts in Africa: Meaning, causes, impact and solutions. Africa Research Review: An International Multi-Disciplinary Journal, *4*(4).
  37. Fang, X., Siddharth, K., McLoughlin, C., & Yenice, M. (2020). The economic consequences of conflict in Sub-Saharan Africa.
  38. Palik, J., Obermeier, A. M., & Rustad, S. A. (2022). Conflict trends in Africa 1989-2021.
  39. ACCORD. (2019). Terrorism in Africa. Conflict Trends 2019/1. https://www.accord.org.za/conflict-trends-issue/2019-1/
  40. Tasamba, J. (2023). Burkina Faso most affected African country by terrorism: Reporthttps://www.aa.com.tr/en/africa/burkina-faso-most-affected-african-country-by-terrorism-report/2857969
  41. Institute of Security Studies - PSC Report. (2022). African conflicts to watch in 2022.
  42. Azama, S. (2015). A critical analysis of Boko Haram Insurgencyhttps://apps.dtic.mil/sti/pdfs/AD1038853.pdf
  43. ACLED. (2018). Profile crisis: Boko Haramhttps://acleddata.com/crisis-profiles/
  44. Van Rentergem, T. (2022). Al-Shabaab in Mozambique: Taking stock of an insurgency undercover.
  45. Bartlet, K. (2022). Mozambique's displaced recount brutality of Cabo Delgado insurgents.
  46. Joseph, N. (2023). Cyclones in Southern Africa: Five essential readshttps://theconversation.com/cyclones-in-southern-africa-five-essential-reads-200371
  47. Smith, P. (2023). 20000 people feared dead in Libyan city destroyed by floods.
  48. IFRC. (2023). Libya: Storm Danielhttps://www.ifrc.org/emergency/libya-storm-daniel
  49. Reuters. (2023). Factbox: Cyclone Freddy among Africa's deadliest stormshttps://www.reuters.com/news/picture/factbox-cyclone-freddy-among-africas-dea-idUSKBN2VH11Z
  50. NASA Earth Observatory. (2023). Heavy rains hit the drought-stricken horn of Africa. https://earthobservatory.nasa.gov/images/151208/heavy-rains-hit-drought-stricken-horn-of-africa
  51. UNDRR. (2022). Climate change increased rainfall associated with tropical cyclones hitting highly vulnerable communities in Madagascar, Mozambique and Malawi.
  52. UNICEF. (2022). Tropical Storm Ana wreaks havoc for children in Madagascar, Malawi and Zimbabwe.
  53. Reliefweb. (2022). Cyclone Batsirai leaves people vulnerable to food shortages and malaria. https://reliefweb.int/report/madagascar/cyclone-batsirai-leaves-people-vulnerable-food-shortages-and-malaria
  54. Oxfam. (2019). Cyclone Idai in Zimbabwe: An analysis of policy implications for post-disaster risk management.
  55. Reliefweb. (2019). Tropical Cyclone Idai-March 2019https://reliefweb.int/disaster/tc-2019-000021-moz
  56. Maedhbh, M. (2016). El Niño drives crisis in Southern and Eastern Africahttps://www.concern.net/news/el-nino-drives-crisis-in-south-and-east-africa
  57. National Oceanic & Atmospheric Administration (NOAA). (2023). What are El Niño & La Nina.
  58. WFP. (2016). Implications of El Nino in Southern Africa from a food and nutrition security perspective.
  59. Harvey, F. (2023). Human-driven climate crisis fuelling Horn of Africa drought study. https://www.theguardian.com/environment/2023/apr/27/human-driven-climate-crisis-fuelling-horn-of-africa-drought-study
  60. WHO. (2023). Drought and food insecurity in the Greater Horn of Africahttps://www.who.int/emergencies/situations/drought-food-insecurity-greater-horn-of-africa
  61. The Guardian. (2023). Human-driven climate crisis fuelling the Horn of Africa. https://www.theguardian.com/environment/2023/apr/27/human-driven-climate-crisis-fuelling-horn-of-africa-drought-study
  62. Brandt, M. (2011). Seismic hazard in South Africa. Council for a Geo Science report.
  63. Kebede, F., & Kulhaneck. (1991). Recent seismicity of East African rift system and its implications. Physics of the Earth and Planetary Interiors.
  64. Wafula, D. M. (2011). Impacts of the tectonic earthquake in the western rift valley of Africa on the volcanic activity of Nyiragongo. Asian Journal of Scientific Research.
  65. Lau, C., O'Murchu, S. F., Raine, A., Vogt, A., & Meyer, M. (2023). 9 September, 2023 Earthquake hits Morocco.
  66. The Guardian. (2023). Morocco earthquake: At least 2000 dead and thousands more injured.
  67. Khalil, A. E., Deif, A., & Abdel, H. E. (2015). Seismic hazard assessments at Islamic Cairo, Egypt. Journal of Earth Sciences.
  68. Samai, S., Idres, M., Boughacha, M. S., Bezzeghoud, M., & Borges, J. F. (2017). A structural scheme, proposal derived from geophysical data in the epicentre area of the Boumerdes, Algeria.
  69. Fontijn, K. (2018). Tracking the hazards and benefits of volcanoes in East Africahttps://theconversation.com/tracking-the-hazards-and-benefits-of-volcanoes-in-east-africa-106322
  70. UNICEF. (2021). Nyiragongo volcano eruption: The aftermathhttps://www.unicef.org/drcongo/en/stories/nyiragongo-volcano-eruption-the-aftermath
  71. Pease, R. (2020). Lava lake rises at dangerous African volcanohttps://pubmed.ncbi.nlm.nih.gov/33060337/
  72. NASA Earth Observatory. (2004). Congo volcanoes erupt. Mt Nyamulagira is Africa's most active volcano.
  73. Royal Belgian Institute for Space Aeronomy. (2022). Africa's most dangerous volcano, may erupt without clear precursory signs.
  74. De Souza, A., Tumuhimbise, A., Mensaque, B. L., & Ogunyale, K. (2023). Wildfire hotspots in Africa and Latin America are facing challenges.
  75. Al Jazeera. (2023). Deadly wildfires raging across Algeriahttps://www.aljazeera.com/gallery/2023/7/25/deadly-wildfires-raging-across-algeria
  76. Harper, D. (2023). North Africa heatwave: Wildfires kill dozens and force over 1,500 people to evacuate.
  77. Reliefweb. (2022). Central African Republic: Fires: February 2022https://reliefweb.int/disaster/fr-2022-000179-caf
  78. IFRC. (2022). Central African Republic: Fires and strong winds in Bakala emergency plan of action.
  79. France 24. (2022). Scenes of devastation as deadly wildfires ravage Algeriahttps://www.france24.com/en/africa/20220818-scenes-of-devastation-as-deadly-wildfires-ravage-algeria
  80. Jones, S. (2022). Thousands evacuated as heat caused wildfires in Europe and North Africa.
  81. Donnefield, Z. (2019). What is driving Sub-Saharan Africa's rapid rise in drug use?
  82. WHO. (2021). Substance abusehttps://www.afro.who.int/health-topics/substance-abuse
  83. Mandura, T. (2023). Zimbabwe battling growing drug addiction among youthshttps://www.trtafrika.com/africa/zimbabwe-battling-growing-drug-addiction-among-youth-12079529
  84. Swansea University. (2014). Global drug policy observatory, country snapshots: Drugs in ZimbabweGdpo.swan.ac.uk
  85. Koigi, B. (2021). Induced drug abuse rise in Africahttps://www.fairplanet.org/story/cases-of-covid-19-induced-drug-abuse-rise-in-africa/
  86. Rehab helper. (2023). How does drug abuse and addiction affect society in South Africahttps://rehabhelper.co.za/blog/how-does-drug-abuse-and-addiction-affect-society-in-south-africa/
  87. Ambali, A., Ewokor, C., & Davies, A. (2023). Kwara boat accident: 100 dead and more missing in Nigeria.
  88. Muslim Network T.V. (2023). 145 people feared dead after Congo boat accidenthttps://www.muslimnetwork.tv/145-people-feared-dead-after-congo-boat-accident/
  89. TRT World. (2023). At least 145 people feared dead in the Democratic Republic of Congo boat tragedy. https://www.trtworld.com/africa/at-least-145-people-feared-dead-in-drc-boat-tragedy-64691
  90. France 24. (2023). Dozens of migrants killed in latest boat disaster off Tunisiahttps://www.france24.com/en/africa/20230326-at-least-19-african-migrants-killed-in-latest-migrant-boast-disaster-off-tunisia
  91. News 24. (2022). 76 people were killed in a Nigeria boat accidenthttps://www.news24.com/news24/africa/news/76-people-killed-in-nigeria-boat-accident-20221010
  92. Magdy, S. (2023). United Nations says at least 55 migrants drowned in a shipwreck off Libya.
  93. UNICEF. (2021). 16 children and 27 adults dead off the coast of Djibouti after migrant boat capsizes.
  94. Nshimbi, C. C. (2021). Southern African countries will only manage disasters if they work together.
  95. African Union. (2023). Building testing capacity for epidemic-prone diseases.
  96. Miller, N. (2022). The animals that detect disastershttps://www.preventionweb.net/news/animals-detect-disasters
  97. Saul, T. (2023). A wilder view: Can animals sense oncoming natural disastershttps://www.kpax.com/news/a-wilder-view/a-wilder-view-can-animals-sense-oncoming-natural-disasters
  98. PBS. (2008). Can animals predict disasters? Tall tales or truehttps://www.pbs.org/wnet/nature/can-animals-predict-disaster-tall-tales-or-true/131/
  99. Bailey, R. (2019). Can animals sense natural disasters? https://www.thoughtco.com/can-animals-sense-natural-disasters-373256
  100. Averett, N. (2020). Do animals really anticipate earthquakes? Sensors hit; they dohttps://www.scientificamerican.com/article/do-animals-really-anticipate-earthquakes-sensors-hint-they-do/

6. Management of Major Insect Pests of Ash Gourd, Benincasa hispida Thunb. with Traps, Biopesticides and Newer Insecticides

Authors: Krishnaveni Mariappan; Ravi Madhaiyan; Allwin Loveson; Suvathi Balu

Keywords: Ash gourd, Insect pests, Sticky traps, Biopesticides, Newer molecules, Benincasa hispida, IPM, Botanicals, Myzus persicae, Aulacophora foveicollis, Diaphania indica.

Page No: 50-58

DIN IJOEAR-JUL-2026-6
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Abstract

Ash gourd, Benincasa hispida Thunb., is an important vegetable crop, cultivated globally for its medicinal values, and its production is hampered by many insect pests. Therefore, field experiments were conducted to study the efficacy of sticky traps, botanicals and organic pesticides, biopesticides, and newer insecticides against major pests of ash gourd in the farmer's holdings at Kaliyavur village of Thoothukudi district, Tamil Nadu, India during Rabi 2018. The results revealed that cylindrical white sticky traps attracted more number of alate forms of Myzus persicae Sulzer, and cylindrical yellow sticky traps attracted more number of adults of Liriomyza trifolii Burgess. Azadirachtin 3000 ppm at 2 mL/L was effective in reducing the insect pests. Beauveria bassiana formulation at 2 g/L was effective against Myzus persicae Sulzer and Aulacophora foveicollis Lucas. Diaphania indica Saunders was controlled by the application of Bacillus thuringiensis at 2 g/L. Newer insecticide molecules were very prominent in managing the major insect pests of ash gourd. The findings of the study will be helpful for the effective management of insect pests of ash gourd.

Keywords: Ash gourd, Insect pests, Sticky traps, Biopesticides, Newer molecules, Benincasa hispida, IPM, Botanicals, Myzus persicae, Aulacophora foveicollis, Diaphania indica.

References
  1. Arida, G. S., Punzal, B. S., Shepard, B. M., & Rajotte, E. G. (2013). Sticky board traps for managing leafminer, Liriomyza trifolii (Burgess) (Diptera: Agromyzidae), infestation in onion (Allium cepa Linn.). Philippine Entomologist, *27*(2), 109-119.
  2. Atwal, A. S. (1993). Agriculture pests of India and South East Asia. Kalyani Publishers.
  3. Azmi, M. A., Naqvi, S. N., Azmi, M. A., & Aslam, M. (2006). Effect of pesticide residues on health and different enzyme levels in the blood of farm workers from Gadap (rural area) Karachi-Pakistan. Chemosphere, *64*(10), 1739-1744.
  4. Christopher Cutler, G., Ramanaidu, K., Astatkie, T., & Isman, M. B. (2009). Green peach aphid, Myzus persicae (Hemiptera: Aphididae), reproduction during exposure to sublethal concentrations of imidacloprid and azadirachtin. Pest Management Science, *65*(2), 205-209.
  5. DES. (2019). Area, production and productivity of Ash gourd (vegetables) by district-wise in Tamil Nadu for year 2016-17. Directorate of Economics and Statistics, Government of India.
  6. Dhillon, M. K., Singh, R., Naresh, J. S., & Sharma, H. C. (2005). The melon fruit fly, Bactrocera cucurbitae: A review of its biology and management. Journal of Insect Science, *5*(1), 40.
  7. Elango, K., Sridharan, S., Saravanan, P. A., & Balakrishnan, S. (2017). Relative performance of different colour laden sticky traps on the attraction of sucking pests in pomegranate. International Journal of Current Microbiology and Applied Sciences, *6*(11), 2997-3004. https://doi.org/10.20546/ijcmas.2017.611.350
  8. El-Salam, A. M. E., Salem, H. A., & Salem, S. A. (2013). Biocontrol agents against the leafminer, Liriomyza trifolii in fababean fields. Archives of Phytopathology and Plant Protection, *46*(9), 1054-1060.
  9. El-sayed, W. (2013). Field evaluation of plant extracts and certain insecticides against Bemisia tabaci (Gennadius) on tomato plants and Myzus persicae (Sulzer) on pepper plants. Journal of Applied Sciences Research, *9*(3), 2372-2377.
  10. Gahlot, N., Kumar, P., Choudhary, A. K., & Gangwar, B. (2024). Performance of management of the red pumpkin beetle (Aulacophora foveicollis) Lucas on bottle gourd by different bio-pesticides. Uttar Pradesh Journal of Zoology, *45*(17), 332-336. https://doi.org/10.56557/upjoz/2024/v45i174376
  11. Gavkare, O., Kumar, S., Sharma, N., & Sharma, P. L. (2013). Evaluation of some novel insecticides against Myzus persicae (Sulzer). The Bioscan, *8*(3), 1119-1121.
  12. Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research (2nd ed.). John Wiley & Sons.
  13. Grupe, B., Dieckhoff, C., & Meyhöfer, R. (2023). Keep an eye on natural enemies: What Aphidius on sticky traps tells us about aphid pest population dynamics. Entomologia Experimentalis et Applicata, *171*(10), 722-731. https://doi.org/10.1111/eea.13360
  14. Halder, J., Kodandaram, M. H., & Rai, A. B. (2011). Differential responses of major vegetable aphids to newer insecticide molecules. Vegetable Sciences, *38*(2), 191-193.
  15. Jacob, J. R., Girija, D., Mathew, M. P., & Gopal, K. S. (2019). Evaluation of Bacillus thuringiensis isolates against Diaphania indica (Saund.) (Lepidoptera: Crambidae). Journal of Tropical Agriculture, *57*(1), 1-9.
  16. Jasrotia, P., Yadav, J., Singh, B., Patil, S. D., Kumar, S., & Singh, G. P. (2022). Efficiency of sticky traps for monitoring aphids in wheat under North-Western Plains and Peninsular zones of India. Journal of Environmental Biology, *43*(6), 794-800. https://doi.org/10.22438/jeb/43/6/MRN-1952
  17. Joseph, A., & Sudharma, K. (2016). Evaluation of bioassay of entomopathogenic fungi, Beauveria bassiana (Bals.) Vuill and Metarhizium anisopliae (Metchnikoff) Sorokin on red pumpkin beetle, Aulacophora foveicollis Lucas. International Journal of Agriculture Innovations and Research, *4*(5), 917-920.
  18. Khan, S., Guo, L., Shi, H., Mijit, M., & Qiu, D. (2012). Bioassay and enzymatic comparison of six entomopathogenic fungal isolates for virulence or toxicity against green peach aphids Myzus persicaeAfrican Journal of Biotechnology, *11*(77), 14193-14203.
  19. Nagaraju, M. C., Nadagouda, S., Hosamani, A. C., & Hurali, S. (2018). Field evaluation of insecticides for the management of cucumber moth, Diaphania indica (Saunders) (Lepidoptera: Crambidae) on bitter gourd. Journal of Entomology and Zoology Studies, *6*(2), 79-82.
  20. Patel, D. R., Patel, R. M., Patel, P. H., & Dabhi, M. V. (2021). Evaluation of different botanicals against red pumpkin beetle, Aulacophora foveicollis Lucas infesting cucumber. International Journal of Current Microbiology and Applied Sciences, *10*(2), 3133-3140. https://doi.org/10.20546/ijcmas.2021.1002.343
  21. Soumya, K., Visalakshy, P. G., Swathi, C., Krishnamoorthy, A., & Gopalakrishna Pillai, K. (2017). Integrated pest management of melon borer, Diaphania indica (Lepidoptera: Pyralidae) in bittergourd. Journal of Biological Control, *31*(4), 240-244.
  22. Sretenovic, M., Miletic, N., & Tamas, N. (2019). Efficacy of different insecticides for the control of green peach aphid (Myzus persicae Sulzer) in nectarine orchards. Pakistan Journal of Agricultural Sciences, *56*(3), 661-668. https://doi.org/10.21162/PAKJAS/19.8690
  23. Tamilnayagan, T., Suganthy, M., Ganapathy, N., Renukadevi, P., & Malathi, V. G. (2017a). Population dynamics of defoliator pests and two spotted spider mite of ash gourd. International Journal of Entomology and Zoology Studies, *5*(3), 293-296.
  24. Tamilnayagan, T., Suganthy, M., Ganapathy, N., Renukadevi, P., & Malathi, V. G. (2017b). Seasonal abundance of sucking pests of ash gourd (Benincasa hispida) (Thunb.) Cogn.) in Tamil Nadu. International Journal of Entomology and Zoology Studies, *5*(3), 430-433.
  25. Tandon, P., & Sirohi, A. (2009). Laboratory assessment of the repellent properties of ethanolic extracts of four plants against Raphidopalpa foveicollis Lucas (Coleoptera: Chrysomelidae). International Journal of Sustainable Crop Production, *4*(2), 1-5.
  26. Tindall, H. D. (1986). Vegetables in the tropics. Macmillan Education Ltd.

7. Nanotechnology: Current Applications, Safety Issues and Future Prospects in Veterinary Pharmacology and Toxicology

Authors: P. Sabarideepa; V.Ramakrishnan; V.Suresh Kumar; S.Malmarugan; S.C.Edwin

Keywords: Nanotechnology; Veterinary pharmacology; Veterinary toxicology; Nanocarriers; Drug delivery systems; Nanotoxicology; Nanomedicine; Targeted drug delivery; Veterinary therapeutics; Nanosafety; Environmental toxicity.

Page No: 59-71

DIN IJOEAR-JUL-2026-7
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Abstract

Nanotechnology has emerged as one of the most promising advancements in modern pharmaceutical sciences due to its ability to manipulate materials at the nanoscale and enhance their interaction with biological systems. In veterinary pharmacology and toxicology, nanotechnology offers innovative solutions to overcome the limitations associated with conventional drug delivery systems, such as poor bioavailability, lack of target specificity, rapid elimination, systemic toxicity, and drug residue concerns in food-producing animals. Nanotechnology-based drug delivery systems improve therapeutic efficacy by enhancing drug solubility, stability, controlled release, and targeted delivery.

Various nanocarriers, including liposomes, polymeric nanoparticles, solid lipid nanoparticles, nanoemulsions, dendrimers, quantum dots, magnetic nanoparticles, nanoshells, and nanopores, have shown significant applications in veterinary therapeutics, diagnostics, oncology, vaccine delivery, and infectious disease management. These nanosystems improve the pharmacokinetic and pharmacodynamic properties of drugs while minimizing adverse effects.

Despite their advantages, nanotechnology-based products raise important safety and toxicological concerns. The unique physicochemical properties of nanoparticles may result in organ toxicity, immunotoxicity, reproductive toxicity, oxidative stress, and environmental hazards. Species-specific responses and long-term exposure effects further complicate safety evaluation in veterinary applications. Therefore, comprehensive nanotoxicological assessment and regulatory oversight are essential before widespread adoption.

The future prospects of nanotechnology in veterinary pharmacology and toxicology include the development of smart nanocarriers, nano-theranostics, nano-phytogenics, precision veterinary medicine, and environmentally safer nanomaterials. This review consolidates findings from multiple scientific studies to present a comprehensive overview of the current applications, safety issues, and future prospects of nanotechnology in veterinary pharmacology and toxicology (Alivisatos, 2004; McNeil, 2009; Patra et al., 2018).

Keywords: Nanotechnology; Veterinary pharmacology; Veterinary toxicology; Nanocarriers; Drug delivery systems; Nanotoxicology; Nanomedicine; Targeted drug delivery; Veterinary therapeutics; Nanosafety; Environmental toxicity.

References
  1. Alghuthaymi, M. A., Hassan, A. A., Kalia, A., Sayed El Ahl, R. M., El Hamaky, A. A., Oleksak, P., ... & Abd-Elsalam, K. A. (2021). Antifungal nano-therapy in veterinary medicine: Current status and future prospects. Journal of Fungi, *7*(7), 494.
  2. Alivisatos, A. P. (2004). The use of nanocrystals in biological detection. Nature Biotechnology, *22*(1), 47-52.
  3. Bilgili, A., & Uysal, M. H. (2019). Use areas of nanotechnology in veterinary medicine. Journal of Food Science and Engineering, *9*, 201-216.
  4. Carvalho, S. G., Rodrigues, L. R., & Lima, E. (2020). Advances and challenges in nanocarriers for veterinary nanomedicines. Journal of Controlled Release, *327*, 555-568.
  5. Chrishtop, V. V., Mironov, V. A., Prilepskii, A. Y., Nikonorova, V. G., & Vinogradov, V. V. (2021). Nanosafety versus nanotoxicology: Adequate animal models for assessment. Toxicology, *462*, 152957.
  6. Dhakad, G., Aich, R., Kushwah, M. S., & Yadav, J. S. (2017). Nanotechnology: Trends and future prospective. Global Journal of Bio-Science and Biotechnology, *6*(3), 548-553.
  7. Feynman, R. P. (1960). There's plenty of room at the bottom. Engineering and Science, *23*(5), 22-36.
  8. Hill, E. K., & Li, J. (2017). Current and future prospects for nanotechnology in animal production. Journal of Animal Science and Biotechnology, *8*(1), 26.
  9. Ianiski, L. B., Faccin-Galhardi, L. C., & Gonçalves, J. L. (2022). Nanotechnology in veterinary medicine: Applications and perspectives. Pesquisa Veterinária Brasileira, *42*, e06944.
  10. McNeil, S. E. (2009). Nanotechnology for the biologist. Journal of Leukocyte Biology, *85*(4), 561-564.
  11. Mweetwa, L. (2022). Past, present and future perspective of application of nanobiotechnology in pharmacology and innovative therapeutics. Medical Research Archives.
  12. Patel, K., Patel, M., Patel, N., Patel, D., & Patel, B. (2017). Nanotechnology-based drug delivery systems in veterinary medicine. Veterinary World, *10*(9), 1051-1057.
  13. Patra, J. K., Das, G., Fraceto, L. F., Campos, E. V. R., Rodriguez-Torres, M. P., Acosta-Torres, L. S., ... & Shin, H. S. (2018). Nano based drug delivery systems: Recent developments and future prospects. Journal of Nanobiotechnology, *16*, 71.
  14. Prasad, R. D., Charmode, N., Shrivastav, O. P., Prasad, S. R., Moghe, A., Sarvalkar, P. D., & Prasad, N. R. (2021). A review on concept of nanotechnology in veterinary medicine. ES Food & Agroforestry, *4*(5), 28-60.
  15. Sapino, S., Chirio, D., Peira, E., & Gallarate, M. (2022). Nanocarriers in veterinary medicine: A challenge for translation. Pharmaceutics, *14*(1), 45.
  16. Singh, A. V. (2019). Review of emerging concepts in nanotoxicology. Nanotoxicology, *13*(1), 1-23.
  17. Taniguchi, N. (1974). On the basic concept of nanotechnology. In Proceedings of the International Conference on Production Engineering (pp. 18-23). Tokyo, Japan.
  18. Trif, E., Cerbu, C., Olah, D., Zăblău, S. D., Spînu, M., Potârniche, A. V., & Brudașcă, F. (2023). Old antibiotics can learn new ways: A systematic review of florfenicol use in veterinary medicine and future perspectives using nanotechnology. Animals, *13*(10), 1695.
  19. Woldeamanuel, K. M., Kurra, F. A., & Roba, Y. T. (2021). A review on nanotechnology and its application in modern veterinary science. International Journal of Nanomaterials, Nanotechnology and Nanomedicine, *7*(1), 026-031.
  20. Youssef, F. S., El-Banna, H. A., Elzorba, H. Y., & Galal, A. M. (2019). Application of some nanoparticles in the field of veterinary medicine. International Journal of Veterinary Science and Medicine, *7*(1), 78-93.
  21. Zhou, Y., Guo, L., Dai, G., Li, B., Bai, Y., Wang, W., & Zhang, J. (2024). An overview of polymeric nanoplatforms to deliver veterinary antimicrobials. Nanomaterials, *14*(4), 341.

8. Evaluation of Natural Materials and Edible Coatings for Ripening Regulation and Shelf-Life Extension of Banana (Musa spp.) and Papaya (Carica papaya L.)

Authors: Spoorthy R; Hari kumar M.V; Manoj D.N; Prashanth S J; Ajith. S

Keywords: Banana, Papaya, Edible coating, Calcium chloride, Sodium alginate, Shelf life, Postharvest, Ripening regulation, Ethylene management, Climacteric fruits, Tropical fruits.

Page No: 72-77

DIN IJOEAR-JUL-2026-9
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Abstract

Fruit ripening is a vital physiological process that enhances the color, flavor, texture, and nutritional quality of climacteric fruits such as banana (Musa spp.) and papaya (Carica papaya L.). However, uncontrolled ripening accelerates senescence, leading to substantial postharvest losses. The present study aimed to evaluate simple, safe, and economical methods for accelerating (forward ripening) and delaying (reverse ripening) fruit ripening under ambient storage conditions. Mature, uniform, and defect-free banana and papaya fruits were subjected to different forward ripening treatments, including paper wrapping, banana leaf covering, plastic cover storage, and storage with ripened fruits to promote natural ethylene accumulation. Reverse ripening treatments consisted of coatings containing calcium chloride and sodium alginate at different concentrations, while potassium permanganate was evaluated as an ethylene scavenger. Fruits were stored at room temperature and monitored for seven days. Quality attributes, including total soluble solids (TSS), physiological weight loss, color development, and texture, were assessed using standard analytical procedures.

The results demonstrated that forward ripening treatments accelerated uniform ripening and improved market readiness through enhanced ethylene accumulation. In contrast, calcium chloride–sodium alginate coatings effectively delayed ripening; the 3% calcium chloride–sodium alginate coating extended ripening to 9 days while reducing physiological weight loss to 7 g, maintained firmness, and preserved fruit quality during storage. Potassium permanganate further contributed to extending shelf life by reducing ethylene concentration.

The study concludes that natural ripening techniques and edible coating treatments provide effective, low-cost, and safe alternatives for ripening management. These approaches have practical applications for farmers, retailers, and fruit industries by reducing postharvest losses, extending shelf life, and improving the marketability and quality of climacteric fruits.

Keywords: Banana, Papaya, Edible coating, Calcium chloride, Sodium alginate, Shelf life, Postharvest, Ripening regulation, Ethylene management, Climacteric fruits, Tropical fruits.

References
  1. Food and Agriculture Organization of the United Nations. (2023). The state of food and agriculture 2023. FAO.
  2. Yahia, E. M., García-Solís, P., & Celis, M. E. M. (2019). Contribution of fruits and vegetables to human nutrition and health. In E. M. Yahia (Ed.), Postharvest physiology and biochemistry of fruits and vegetables (pp. 19-45). Woodhead Publishing.
  3. Wills, R. B. H., & Golding, J. B. (2016). Postharvest: An introduction to the physiology and handling of fruit and vegetables (6th ed.). CABI.
  4. Paul, V., Pandey, R., & Srivastava, G. C. (2023). Role of ethylene in fruit ripening and postharvest management of climacteric fruits. Journal of Food Science and Technology, *60*(5), 1453-1467.
  5. Bisen, A., & Pandey, S. K. (2008). Natural methods of fruit ripening and postharvest handling of tropical fruits. Indian Horticulture, *53*(2), 18-22.
  6. Hasan, M. U., Riaz, R., Malik, A. U., Khan, A. S., Anwar, R., & Rehman, R. N. U. (2021). Recent advances in edible coatings for extending the postharvest shelf life of fresh fruits. Food Reviews International, *37*(5), 435-450.
  7. Dhall, R. K. (2020). Advances in edible coatings for fresh fruits and vegetables: A review. Critical Reviews in Food Science and Nutrition, *60*(3), 435-450.
  8. Baldwin, E. A., Hagenmaier, R., & Bai, J. (2022). Edible coatings and films to improve food quality (2nd ed.). CRC Press.
  9. Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research (2nd ed.). John Wiley & Sons.
  10. AOAC International. (2019). Official methods of analysis of AOAC International (21st ed.). AOAC International.
  11. Ali, A., Maqbool, M., Ramachandran, S., & Alderson, P. G. (2022). Natural packaging materials and biodegradable films for postharvest preservation of fresh fruits and vegetables: A review. Food Packaging and Shelf Life, *31*, 100787.
  12. Kader, A. A. (2021). Postharvest technology of horticultural crops: Recent advances and future challenges. Horticulturae, *7*(8), 206.

9. Analysis of the Dynamics of Forest Landscape Fragmentation in Western Madagascar under the Influence of Migration

Authors: Fabiola Fidisoa Viraina; Herizo Tiana Andrianandrasana; Claude Jacquot Ralazampirenena; Marrino Falitiana Rakotoarisoa; Carles Olivier Tatafasa; Luciano Willy Nathoo Veriza; Lily-Arison Rene De Roland

Keywords: Menabe Antimena Protected Area, Migration, Anthropogenic pressure, Fragmentation, Conservation, Forest fragmentation, Deforestation, Remote sensing, Landscape ecology, Madagascar, Dry forest, Climate-induced migration, Protected area management.

Page No: 78-96

DIN IJOEAR-JUL-2026-13
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Abstract

Economic activities by migrant populations lead to changes in landscape structure and land use in non-forested areas. This article aims to analyse the spatial and temporal dynamics of forest fragmentation and to examine the influence of migration on the configuration of the forest landscape within the Menabe Antimena protected area between 2017 and 2022. The methods used include a diachronic analysis of satellite imagery using remote sensing. Following canopy classification, land-use classes were categorised as forest or non-forest. The results reveal that forest fragmentation shows a generalised increase of 13.94% within the protected area and 25.05% at its periphery, with an overall peak observed in 2022. Furthermore, the configuration of forest fragments is significantly correlated with migration inflows. The landscape is dominated by micro-fragments covering an area of less than 20 ha, which account for between 98.86% (16,197 out of 16,383 fragments) and 99.18% (20,622 out of 20,792 fragments) of the total within the protected area; and 99.08% (11,607 out of 11,715 fragments) to 99.30% (12,226 out of 12,312 fragments) outside the protected area. At the same time, fragment density increased from 2.32 to 3.92 fragments/ha, resulting in a significant change in the fragmentation index (from 0.60 to 0.87) for the 20–50 ha class. These results make a major contribution to the overhaul of national conservation strategies by demonstrating the need to incorporate internal population flows into protected area management plans.

Keywords: Menabe Antimena Protected Area, Migration, Anthropogenic pressure, Fragmentation, Conservation, Forest fragmentation, Deforestation, Remote sensing, Landscape ecology, Madagascar, Dry forest, Climate-induced migration, Protected area management.

References
  1. Achard, F., Eva, H. D., Mayaux, P., Stibig, H. J., & Belward, A. (2004). Improved estimates of net carbon emissions from land cover change in the tropics for the 1990s. Global Biogeochemical Cycles, *18*(2). https://doi.org/10.1029/2003GB002142
  2. Agrawal, A. (2018). Réévaluation de l'importance relative de la pression démographique, des forces du marché et des institutions sur l'état des forêts, à partir d'une analyse des conseils villageois de gestion forestière du Kumaon, dans l'Himalaya moyen indien [Research report]. University of Florida.
  3. Ahmed, H., Jallat, H., Hussain, E., Saqib, N. U., Saqib, Z., Khokhar, M. F., et al. (2023). Quantitative assessment of deforestation and forest degradation in Margalla Hills National Park (MHNP): Employing landsat data and socio-economic survey. Forests, *14*, 201. https://doi.org/10.3390/f14020201
  4. Andrianambinina, F. O. D., Ganzhorn, J. U., Waeber, P. O., & Wilmé, L. (2025). Complex deforestation patterns in and around the protected areas of Madagascar from 2015 to 2023. Land, *14*(4), 698. https://doi.org/10.3390/land14040698
  5. Andrianambinina, F. O. D., Rafanoharana, S. C., Rasamuel, H. A. T., Waeber, P. O., Ganzhorn, J. U., & Wilmé, L. (2024). Decrease of deforestation in protected areas of Madagascar during the Covid-19 years. Madagascar Conservation & Development, *18*(1), 15-21. https://doi.org/10.4314/mcd.v18i1.2
  6. Andrianandrasana, H. T., Jones, N., Viraina, F. F., Malesios, C., Campera, M., Sama, Z., Long, P. R., Panichelli-Batalla, S., Richardson, N. M., & Savage, J. (2025). Links between poverty, climate-induced migration and deforestation in western Madagascar. Environmental Development, *56*. https://doi.org/10.1016/j.envdev.2025.101284
  7. Aslam, M., & Fazal, S. (2025). Exploring the impact of land fragmentation on the performance of agriculture: A systematic review. Discover Agriculturehttps://doi.org/10.1007/s44279-025-00207-6
  8. Asner, G. P., Rudel, T. K., Aide, T. M., Defries, R., & Emerson, R. (2009). A contemporary assessment of change in humid tropical forests. Conservation Biology, *23*(6), 1386-1395. https://doi.org/10.1111/j.1523-1739.2009.01333.x
  9. Aubert, S., Rambintsaotra, S., & Razafiarijaona, J. (2013). L'insécurité foncière dans et autour des Aires Protégées de Madagascar : un obstacle à surmonter pour la conservation de la biodiversité et le développement rural. Développement durable et territoires, *4*(1). https://doi.org/10.4000/developpementdurable.9661
  10. Bakdash, J. Z., & Marusich, L. R. (2017). Repeated measures correlation. Frontiers in Psychology, *8*, 456. https://doi.org/10.3389/fpsyg.2017.00456
  11. Bechard, L. (2018). Les migrations environnementales en 2018 : l'agenda 2030 des Nations Unies et la responsabilité des pays du Nordhttp://hdl.handle.net/11143/12844
  12. Berenger, E., & Morrissey, S. (2022). Successful 20 years of community forest management in Guatemala informs an Integrated Community Forest Management pathway to support scaling. In XV World Forestry Congress, 2-6 May 2022, Seoul, Republic of Korea. FAO. https://openknowledge.fao.org/handle/20.500.14283/y2661e
  13. Bogaert, J., & Mahamane, A. (2005). Ecologie du paysage : cibler la configuration et l'échelle spatiale. Annales des Sciences Agronomiques, *7*(1), 39-68. https://doi.org/10.4314/asab.v7i1.43277
  14. Bogaert, J., Ceulemans, R., & Salvador-Van Eysenrode, D. (2004). Decision tree algorithm for detection of spatial processes in landscape transformation. Environmental Management, *33*, 62-73. https://doi.org/10.1007/s00267-003-0027-0
  15. Bogaert, J., & Hong, S. K. (2004). Landscape ecology: Monitoring landscape dynamics using spatial pattern metrics. In S. K. Hong et al. (Eds.), Ecological issues in a changing world (pp. 1-15). Springer. https://doi.org/10.1007/978-1-4020-2689-8_8
  16. Brou, Y. T. (2018). Pressions agro-démographiques dans les espaces forestiers protégés de Madagascar: l'exemple du Parc National d'Ankarafantsika. Carnets de Recherches de l'océan Indien, (2). https://hal.archives-ouvertes.fr/hal-02474941
  17. Brown, K., & Pearce, D. W. (2023). The causes of tropical deforestation: The economic and statistical analysis of factors giving rise to the loss of the tropical forests. Routledge. https://doi.org/10.4324/9781003428190
  18. Bwalya Umar, B., & Kapembwa, J. (2020). Economic benefits, local participation, and conservation ethic in a game management area: Evidence from Mambwe, Zambia. Tropical Conservation Science, *13*, 1940082920971754. https://doi.org/10.1177/1940082920971754
  19. Caron, S., Garvey, S. M., Gewirtzman, J., Schultz, K., Bhatnagar, J. M., Driscoll, C., ... & Templer, P. H. (2023). L'urbanisation et la fragmentation ont des effets opposés sur la disponibilité de l'azote dans les sols des écosystèmes forestiers tempérés. Global Change Biology, *29*(8), 2156-2171. https://doi.org/10.1111/gcb.16611
  20. Collinge, S. K., & Forman, R. T. T. (1998). A conceptual model of land conversion processes: Prediction and evidence from a microlandscape experiment with grassland insect. Oikos, *82*, 66-84. https://doi.org/10.2307/3546918
  21. Congalton, R. G. (1991). A review of assessing the accuracy of classifications of remotely sensed data. Remote Sensing of Environment, *37*(1), 35-46.
  22. Edwards, D. P., Socolar, J. B., Mills, S. C., Burivalova, Z., Koh, L. P., & Wilcove, D. S. (2019). Conservation of tropical forests in the anthropocene. Current Biology, *29*(19), R1008-R1020. https://doi.org/10.1016/j.cub.2019.08.026
  23. FAO & JRC. (2012). Global Forest Land-use Change 1990–2005 (FAO Forestry Paper No. 169). Food and Agriculture Organization of the United Nations and European Commission Joint Research Centre.
  24. Fischer, R., Taubert, F., Müller, M. S., Groeneveld, J., Lehmann, S., Wiegand, T., & Huth, A. (2021). Accelerated forest fragmentation leads to critical increase in tropical forest edge area. Science Advances, *7*(37), eabg7012. https://doi.org/10.1126/sciadv.abg7012
  25. Fitz, J., Adenle, A. A., & Speranza, C. I. (2022). Increasing signs of forest fragmentation in the Cross River National Park in Nigeria: Underlying drivers and need for sustainable responses. Ecological Indicators, *139*, 108943. https://doi.org/10.1016/j.ecolind.2022.108943
  26. Fu, X., Li, Z., Ma, J., Zhou, M., Chen, L., & Peng, J. (2025). Ecosystem resilience response to forest fragmentation in China: Thresholds identification. Journal of Environmental Management, *380*, 125180. https://doi.org/10.1016/j.jenvman.2025.125180
  27. Geldmann, J., Manica, A., Burgess, N. D., Coad, L., & Balmford, A. (2019). A global-scale assessment of changes in human pressure on protected areas. Conservation Biology, *33*(5), 1160-1170.
  28. Goodman, S. M., Raherilalao, M. J., & Wohlhauser, S. (Eds.). (2018). The terrestrial protected areas of Madagascar: Their history, description, and biota (Vol. III: Western and Southwestern Madagascar – synthesis). Association Vahatra.
  29. Hänggli, A., Levy, S. A., Armenteras, D., Bovolo, C. I., Brandão, J., Rueda, X., & Garrett, R. D. (2023). A systematic comparison of deforestation drivers and policy effectiveness across the Amazon biome. Environmental Research Letters, *18*(7), 073001. https://doi.org/10.1088/1748-9326/acd408
  30. Hansen, M. C., Wang, L., Song, X. P., Tyukavina, A., Turubanova, S., Potapov, P. V., & Stehman, S. V. (2020). The fate of tropical forest fragments. Science Advances, *6*(11), eaax8574. https://doi.org/10.1126/sciadv.aax8574
  31. Hansen, M. C., Potapov, P. V., Moore, R., Hancher, M., Turubanova, S. A., Tyukavina, A., et al. (2013). High-resolution global maps of 21st-century forest cover change. Science, *342*, 850-853. https://doi.org/10.1126/science.1244693
  32. Hauer, M. E., Jacobs, S. A., & Kulp, S. A. (2024). Climate migration amplifies demographic change and population aging. Proceedings of the National Academy of Sciences, *121*(3), e2206192119. https://doi.org/10.1073/pnas.2206192119
  33. Hending, D., Randrianarison, H., Andriamavosoloarisoa, N. N. M., Ranohatra-Hending, C., McCabe, G., Cotton, S., & Holderied, M. W. (2025). Impact of forest fragmentation and associated edge effects on tropical forest biodiversity in North West Madagascar, assessed via ecoacoustics. Animal Conservationhttps://doi.org/10.1111/acv.70037
  34. Hending, D., Randrianarison, H., Andriamavosoloarisoa, N. N. M., Ranohatra-Hending, C., Holderied, M., McCabe, G., & Cotton, S. (2023). Forest fragmentation and its associated edge-effects reduce tree species diversity, size, and structural diversity in Madagascar's transitional forests. Biodiversity and Conservation, *32*(10), 3329-3353. https://doi.org/10.1007/s10531-023-02657-0
  35. Hisham, T. W. B., & Hua, A. K. (2026). Spatial analysis of forest fragmentation impacts on ecosystem services in Petaling, Malaysia (2005–2023). Journal of Landscape Ecology, *19*(2). https://doi.org/10.2478/jlecol-2026-0020
  36. Hlovor, A. K. D., Adjonou, K., Dangbo, F. A., Abotsi, K. E., Afelu, B., & Kokou, K. (2021). Dynamique du couvert forestier dans la partie méridionale des Monts Togo, Afrique de l'Ouest. Revue Ivoirienne des Sciences et Technologie, *37*, 300-313.
  37. Holden, S. T., & Otsuka, K. (2014). The roles of land tenure reforms and land markets in the context of population growth and land use intensification in Africa. Food Policy, *48*, 88-97. https://doi.org/10.1016/j.foodpol.2014.03.005
  38. IOM (International Organization for Migration). (2024). Indice de solution et de mobilité - Région Menabe, Madagascar. Institut mondial des données. Matrice de suivi des déplacements.
  39. Jaeger, J. A. G. (2000). Landscape division, splitting index, and effective mesh size: New measures of landscape fragmentation. Landscape Ecology, *15*, 115-130. https://doi.org/10.1023/A:1008129329289
  40. Jallat, H., Khokhar, M. F., Kudus, K. A., Nazre, M., Saqib, N. U., Tahir, U., et al. (2021). Monitoring carbon stock and land-use change in 5000-year-old juniper forest stand of Ziarat, Balochistan, through a synergistic approach. Forests, *12*, 1-15. https://doi.org/10.3390/f12010051
  41. Jaroensutasinee, K., Jaroensutasinee, M., Chuachart, O., & Sparrow, E. (2024). Protected area size affecting habitat fragmentation: A case study of protected areas in Thailand. In Sustainable forest management: Surpassing climate change and land degradation. IntechOpen. https://doi.org/10.5772/intechopen.1004276
  42. Jones, J. P. G., Mandimbiniaina, R., Kelly, R., et al. (2018). Human migration to the forest frontier: Implications for land use change and conservation management. Geo: Geography and Environmenthttps://doi.org/10.1002/geo2.50
  43. Kalinauskas, M., Shuhani, Y., Pinto, L. V., Inácio, M., & Pereira, P. (2024). Mapping ecosystem services in protected areas: A systematic review. Science of the Total Environment, *912*, 169248. https://doi.org/10.1016/j.scitotenv.2023.169248
  44. Kievit, R. A., Frankenhuis, W. E., Waldorp, L. J., & Borsboom, D. (2013). Simpson's paradox in psychological science: A practical guide. Frontiers in Psychology, *4*, 513. https://doi.org/10.3389/fpsyg.2013.00513
  45. Kyaw, K. T. W., Ota, T., Mizoue, N., & Chicas, S. D. (2024). Uncovering the conservation effectiveness of community forests: A case study from Shan State in Myanmar. Biological Conservation, *300*, 110846. https://doi.org/10.1016/j.biocon.2024.110846
  46. Lamy, T., Liss, K., Gonzalez, A., & Bennett, E. (2016). Landscape structure affects the provision of multiple ecosystem services. Environmental Research Letters, *11*(12), 124017. https://doi.org/10.1088/1748-9326/11/12/124017
  47. Laudari, H. K., Sapkota, L. M., Maraseni, T., Subedi, P., Pariyar, S., Kaini, T. R., ... & Volkova, L. (2024). Community forestry in a changing context: A perspective from Nepal's mid-hill. Land Use Policy, *138*, 107018. https://doi.org/10.1016/j.landusepol.2023.107018
  48. Levers, C., Romero-Muñoz, A., Baumann, M., De Marzo, T., Fernández, P. D., Gasparri, N. I., ... & Kuemmerle, T. (2021). Agricultural expansion and the ecological marginalization of forest-dependent people. Proceedings of the National Academy of Sciences, *118*(44), e2100436118. https://doi.org/10.1073/pnas.2100436118
  49. Levick, S. R., & Rogers, K. H. (2011). Context-dependent vegetation dynamics in an African savanna. Landscape Ecology, *26*(4), 515-528. https://doi.org/10.1007/s10980-011-9578-2
  50. Li, D., Yang, Y., Xia, F., Sun, W., Li, X., & Xie, Y. (2022). Exploring the influences of different processes of habitat fragmentation on ecosystem services. Landscape and Urban Planning, *227*, 104544. https://doi.org/10.1016/j.landurbplan.2022.104544
  51. Ma, J., Li, J., Wu, W., & Liu, J. (2023). Global forest fragmentation change from 2000 to 2020. Nature Communications, *14*(1), 3752. https://doi.org/10.1038/s41467-023-39221-x
  52. Mahapatra, S., Majhi, B. K., Sarkar, M. S., Datta, D., Mishra, A. P., & Rathnayake, U. (2025). Understanding forest fragmentation dynamics and identifying drivers for forest cover loss using random forest models to develop effective forest management strategies in North-East India. Results in Engineering, *26*, 104640. https://doi.org/10.1016/j.rineng.2025.104640
  53. Markolf, M., Schäffler, L., & Kappeler, P. M. (2019). Microcebus berthae. The IUCN Red List of Threatened Species. https://www.iucnredlist.org/species/41573/115579496
  54. Marusich, L. R., & Bakdash, J. Z. (2021). rmcorrShiny: A web and standalone application for repeated measures correlation. F1000Research, *10*, 697. https://doi.org/10.12688/f1000research.55027.2
  55. McGarigal, K., & Marks, B. J. (1995). FRAGSTATS: Spatial pattern analysis program for quantifying landscape structure (USDA Forest Service General Technical Report PNW-351). https://doi.org/10.2737/PNW-GTR-351
  56. MEDD (Ministère de l'Environnement et du Développement Durable). (2022). Plan d'Aménagement et de Gestion de l'Aire Protégée Menabe Antimena (2022-2026).
  57. Monmonier, M. S. (1974). Measures of pattern complexity for choroplethic maps. The American Cartographer, *1*(2), 159-169. https://doi.org/10.1559/152304074784107728
  58. Muraoka, K., Jin, S., & Jayne, T. S. (2018). Land access, land rental and food security: Evidence from Kenya. Land Use Policy, *70*, 611-622. https://doi.org/10.1016/j.landusepol.2017.10.045
  59. Muttenzer, F. (2012). Community forest management on the agricultural frontier: Charcoal makers, immigrant associations and land claims in Ankarafantsika, North-West Madagascar. Les Cahiers d'Outre-Mer, *65*(258), 249-272. https://doi.org/10.4000/com.6604
  60. Myers, N., Mittermeier, R., Mittermeier, C., et al. (2000). Biodiversity hotspots for conservation priorities. Nature, *403*, 853-858. https://doi.org/10.1038/35002501
  61. Nicoletti, A., Melde, S., Guadagno, L., & Bilsborrow, R. E. (2023). Human mobility in the context of environmental and climate change: Recent data collection tools from the International Organization for Migration to address key methodological and conceptual issues. International Migration, *61*(5), 47-59. https://doi.org/10.1111/imig.13043
  62. Olofsson, P., Foody, G. M., Herold, M., Stehman, S. V., Woodcock, C. E., & Wulder, M. A. (2014). Good practices for estimating area and assessing accuracy of land change. Remote Sensing of Environment, *148*, 42-54.
  63. Oloukoï, J., Mama, V. J., & Agbo, B. F. (2006). Modélisation de la dynamique de l'occupation des terres dans le département des collines au Bénin. Revue Télédétection, *6*(4), 305-323.
  64. Panigrahi, S., & Vidyarthi, V. K. (2024). Assessing the suitability of McKee et al. (1993) drought severity classification across India. Natural Hazards, *120*(14), 13543-13572. https://doi.org/10.1007/s11069-024-06762-3
  65. Payne, R. (2020). Anova and design (21st ed.). VSN International. https://genstat.kb.vsni.co.uk/wp-content/uploads/sites/2/AnovaGuide.pdf
  66. Peptenatu, D., Andronache, I., Ahammer, H., Radulovic, M., Costanza, J. K., Jelinek, H. F., ... & Newman, E. A. (2023). A new fractal index to classify forest fragmentation and disorder. Landscape Ecology, *38*(6), 1373-1393. https://doi.org/10.1007/s10980-023-01640-y
  67. Perea, M. (2024). Exploring the effects of forest fragmentation on ecosystem health and resilience. Journal of Biodiversity & Endangered Species, *12*, 530. https://doi.org/10.37421/2332-2543.2024.12.530
  68. Puyravaud, J. P. (2003). Standardizing the calculation of the annual rate of deforestation. Forest Ecology and Management, *177*(1-3), 593-596.
  69. Rabemananjara, Z. H. (2014). Migration causing forest degradation in Madagascar: Prevention or adaptation to the effects? Pinnacle Natural Resources & Conservation, *1*(1), 194-201.
  70. Rafanoharana, S. C., Andrianambinina, F. O. D., Rasamuel, H. A., Waeber, P. O., Wilmé, L., & Ganzhorn, J. U. (2024). Projecting forest cover in Madagascar's protected areas to 2050 and its implications for lemur conservation. Oryx, *58*(2), 155-163. https://doi.org/10.1017/S0030605323001175
  71. Rafanoharana, S. C., Andrianambinina, F. O. D., Rasamuel, H. A., Waeber, P. O., Ganzhorn, J. U., & Wilmé, L. (2023). Tree canopy density thresholds for improved forests cover estimation in protected areas of Madagascar. Environmental Research Communications, *5*(7), 071003. https://doi.org/10.1088/2515-7620/ace87f
  72. Rahman, M. F., & Islam, K. (2021). Effectiveness of protected areas in reducing deforestation and forest fragmentation in Bangladesh. Journal of Environmental Management, *280*, 111711. https://doi.org/10.1016/j.jenvman.2020.111711
  73. Rakotoarisoa, S. E., Andriajaona, A., Andriamihajarivo, T., Andriamparany, S., et al. (Eds.). (2025). Madagascar Protected Area Outlook 2025: A conservation assessment of terrestrial Protected Areas in Madagascarhttps://conservationallies.org/outlook/
  74. Rakotonarivo, O. S., Mueller, V., Rakotoarisoa, M., Rakoto Harison, H., & Bell, A. R. (2026). Beyond blame: Migration's limited role in Madagascar's deforestation. Conservation Letters, *19*(1), e70018. https://doi.org/10.1111/con4.70018
  75. Ravonjimalala, H. R. (2019). Modélisation spatiale des changements d'occupation du sol et de la fragmentation de la forêt dense sèche du Sud-ouest de Madagascar : cas de la commune Analamisampy [Doctoral dissertation, Université de Antananarivo].
  76. Rives, F. (2006). Faire le deuil de la forêt primaire pour sauver les forêts ? étude des pratiques et des représentations paysannes de la forêt pour élaborer des stratégies de restauration dans le corridor forestier de Ankeniheny Zahamena est de Madagascar [Doctoral dissertation, ENGREF].
  77. Salvaterra & INDRI. (2017). Analyse des moteurs de déforestation et de dégradation dans les écorégions des forêts humides de l'Est et des forêts sèches de l'Ouest de Madagascar (Rapport de synthèse - Livrable 4). Bureau National de Coordination REDD+ (BNC REDD+), Ministère de l'Environnement, de l'Écologie et des Forêts, République de Madagascar.
  78. Sambiéni, K. R., Toyi, M. S., & Mama, A. (2015). Perception paysanne sur la fragmentation du paysage de la forêt classée de l'Ouémé Supérieur au nord du Bénin. Vertigo, *15*(12). https://doi.org/10.4000/vertigo.16477
  79. Scales, I. R. (2011). Farming at the forest frontier: Land use and landscape change in Western Madagascar, 1896-2005. Environment and History, *17*(4), 499-524. https://doi.org/10.3197/096734011X13150366551481
  80. Scholte, P., & De Groot, W. T. (2010). From Debaste to insight: Three models of immigration to protected areas. Conservation Biology, *24*(2), 630-632. https://doi.org/10.1111/j.1523-1739.2009.01314.x
  81. Senior, R. A., Hill, J. K., & Edwards, D. P. (2019). Global loss of climate connectivity in tropical forests. Nature Climate Change, *9*(8), 623-626. https://doi.org/10.1038/s41558-019-0529-2
  82. Sist, P. (2025). Sustainable management of tropical forests. Éditions Quæ. https://doi.org/10.35690/978-2-7592-4188-0
  83. Sunderlin, W. D., & Pokam, J. (2002). Economic crisis and forest cover change in Cameroon: The roles of migration, crop diversification, and gender division of labor. Economic Development and Cultural Change, *50*(3). https://doi.org/10.1086/342358
  84. Taubert, F., Fischer, R., Groeneveld, J., Lehmann, S., Müller, M. S., Rödig, E., Wiegand, T., & Huth, A. (2018). Global patterns of tropical forest fragmentation. Nature, *554*, 519-522. https://doi.org/10.1038/nature25508
  85. Tchibozo, E. A. M. (2020). Modélisation de la dynamique spatio-temporelle de l'occupation du sol et analyse des changements du territoire de La Lama au Bénin. European Scientific Journal, *16*(6). https://doi.org/10.19044/esj.2020.v16n6p500
  86. Tovondrazane, C. A., et al. (2020). Dynamique spatio-temporelle de succession forestière dans la forêt des Mikea et ses périphériques, Sud-ouest de Madagascar.
  87. Triplet, P. (2009). Manuel de gestion des aires protégées d'Afrique francophone. Awely. https://hal.archives-ouvertes.fr/hal-00669157
  88. Veron, J. (2020). Migrations et changement climatique: Un phénomène aux dimensions incertaines. In T. De Montbrial & D. David (Eds.), Ramses 2021: Le grand basculement ? (pp. 78-83). Institut français des relations internationales. https://doi.org/10.3917/ifri.demon.2020.01.0078
  89. Vieilledent, G., Grinand, C., Rakotomalala, F., Ranaivosoa, R., Rakotoarijaona, J. R., Allnutt, T. F., & Achard, F. (2018). Combining global tree cover loss data with historical national forest cover maps to look at six decades of deforestation and forest fragmentation in Madagascar. Biological Conservation, *222*, 189-197. https://doi.org/10.1016/j.biocon.2018.04.008
  90. Vimal, R., Navarro, L. M., Jones, Y., Wolf, F., Le Moguédec, G., & Réjou-Méchain, M. (2021). The global distribution of protected areas management strategies and their complementarity for biodiversity conservation. Biological Conservation, *256*, 109014. https://doi.org/10.1016/j.biocon.2021.109014
  91. Vinke, K., Rottmann, S., Gornott, C., Zabre, P., Schwerdtle, P. N., & Sauerborn, R. (2022). Is migration an effective adaptation to climate-related agricultural distress in sub-Saharan Africa? Population and Environment, *43*, 319-345. https://doi.org/10.1007/s11111-021-00393-7
  92. Wade, T. G., Riitters, K. H., Wickham, J. D., & Jones, K. B. (2003). Distribution and causes of global forest fragmentation. Conservation Ecology, *7*(2). https://www.jstor.org/stable/26271943
  93. Wainger, L. A., King, D. M., Mack, R. N., Price, E. W., & Maslin, T. (2010). Can the concept ecosystem services be practically applied to improve natural resource management decisions? Ecological Economics, *69*, 978-987. https://doi.org/10.1016/j.ecolecon.2009.12.011
  94. Wilmet, L., Toto Volahy, A., & Hudson, M. (2022). Hypogeomys antimena. The IUCN Red List of Threatened Species. https://doi.org/10.2305/IUCN.UK.2022-1.RLTS.T10714A216087357.fr
  95. Wittemyer, G., Elsen, P., Bean, W. T., Burton, A. C. O., & Brashares, J. S. (2008). Accelerated human population growth at protected area edges. Science, *321*(5885), 123-126.
  96. Yuan, R., Zhang, N., & Zhang, Q. (2024). The impact of habitat loss and fragmentation on biodiversity in global protected areas. Science of the Total Environment, *931*, 173004. https://doi.org/10.1016/j.scitotenv.2024.173004
  97. Zapata, S. V., Vargas, G. A., Castano, E., Munoz, C., Londoño, Y., Zamudio-Mir, M. C., ... & Eslava, A. (2026). Against the odds: Community forestry in the San Lucas Mountains, Colombia. World Development, *198*, 107231. https://doi.org/10.1016/j.worlddev.2025.107231
  98. Zhang, N., Shang, C., Wu, J., & Zhang, Q. (2026). Impacts of surrounding land development on fragmentation within global protected areas. Resources, Conservation and Recycling, *227*, 108749. https://doi.org/10.1016/j.resconrec.2025.108749
  99. Zinner, D., Wygoda, C., Razafimanantsoa, L., Rasoloarison, R., Andrianandrasana, H. T., Ganzhorn, J. U., & Torkler, F. (2014). Analysis of deforestation patterns in the central Menabe, Madagascar, between 1973 and 2010. Regional Environmental Change, *14*(1), 157-166. https://doi.org/10.1007/s10113-013-0475-x
  100. Zou, Y., Crowther, T. W., Smith, G. R., Ma, H., Mo, L., Bialic-Murphy, L., & Zohner, C. M. (2025). Fragmentation increased in over half of global forests from 2000 to 2020. Science, *389*(6765), 1151-1156. https://doi.org/10.1126/science.adr6450.

10. Genomic Selection in Animal Breeding: Principles, Applications, and Future Perspectives

Authors: Shalu Kumari Pathak; Amit Kumar; Vaishali Sah

Keywords: Genomic selection; Genomic estimated breeding value; Genomic prediction; Single nucleotide polymorphism; Livestock breeding; Genomic BLUP; Animal genetics; GBLUP; ssGBLUP; Quantitative genetics; Marker-assisted selection; SNP; Heritability.

Page No: 97-104

DIN IJOEAR-JUL-2026-14
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Abstract

Genomic selection (GS) has transformed modern animal breeding by enabling the prediction of genetic merit using dense genome-wide molecular markers rather than relying solely on pedigree and phenotypic information. Since its conceptual introduction in 2001, GS has become a cornerstone of genetic improvement programs in livestock species, particularly dairy cattle, and has subsequently expanded to beef cattle, sheep, goats, swine, poultry, and aquaculture. The integration of high-density single nucleotide polymorphism (SNP) genotyping with advanced statistical prediction models has substantially increased the accuracy of breeding value estimation, shortened generation intervals, and accelerated rates of genetic gain. Compared with conventional best linear unbiased prediction (BLUP) and marker-assisted selection (MAS), genomic selection captures the combined effects of thousands of loci distributed throughout the genome, making it highly effective for complex quantitative traits governed by many genes of small effect. Recent advances, including single-step genomic BLUP, Bayesian prediction methods, whole-genome sequence analysis, functional genomics, multi-omics integration, artificial intelligence, and precision livestock farming technologies, have further enhanced the scope and efficiency of genomic prediction. These innovations are facilitating simultaneous improvement in productivity, fertility, feed efficiency, disease resistance, animal welfare, and environmental sustainability. Moreover, genomic information is increasingly being integrated with genome editing technologies such as CRISPR to support precision breeding strategies. This review summarizes the historical evolution, fundamental principles, methodological developments, and practical applications of genomic selection in livestock breeding while highlighting emerging innovations and future research directions that are expected to shape next-generation animal improvement programs.

Keywords: Genomic selection; Genomic estimated breeding value; Genomic prediction; Single nucleotide polymorphism; Livestock breeding; Genomic BLUP; Animal genetics; GBLUP; ssGBLUP; Quantitative genetics; Marker-assisted selection; SNP; Heritability.

References
  1. Meuwissen, T. H. E., Hayes, B. J., & Goddard, M. E. (2001). Prediction of total genetic value using genome-wide dense marker maps. Genetics, *157*(4), 1819-1829. https://doi.org/10.1093/genetics/157.4.1819
  2. Schaeffer, L. R. (2006). Strategy for applying genome-wide selection in dairy cattle. Journal of Animal Breeding and Genetics, *123*(4), 218-223. https://doi.org/10.1111/j.1439-0388.2006.00595.x
  3. Meuwissen, T., Hayes, B., & Goddard, M. (2016). Genomic selection: A paradigm shift in animal breeding. Animal Frontiers, *6*(1), 6-14. https://doi.org/10.2527/af.2016-0002
  4. Eggen, A. (2012). The development and application of genomic selection as a new breeding paradigm. Animal Frontiers, *2*(1), 10-15. https://doi.org/10.2527/af.2011-0027
  5. VanRaden, P. M. (2008). Efficient methods to compute genomic predictions. Journal of Dairy Science, *91*(11), 4414-4423. https://doi.org/10.3168/jds.2007-0980
  6. Silva, M. V. B., et al. (2014). The development of genomics applied to dairy breeding. Livestock Science, *166*, 66-75. https://doi.org/10.1016/j.livsci.2014.05.017
  7. Ibtisham, F., Zhang, L., Xiao, M., An, L., Ramzan, M. B., Nawab, A., Zhao, Y., Li, G., & Xu, Y. (2017). Genomic selection and its application in animal breeding. The Thai Journal of Veterinary Medicine, *47*(3), Article 1. https://doi.org/10.56808/2985-1130.2838
  8. Weller, J. I., Ezra, E., & Ron, M. (2017). Invited review: A perspective on the future of genomic selection in dairy cattle. Journal of Dairy Science, *100*(10), 8633-8644. https://doi.org/10.3168/jds.2017-12879
  9. Hill, W. G. (2014). Applications of population genetics to animal breeding, from Wright, Fisher and Lush to genomic prediction. Genetics, *196*(1), 1-16. https://doi.org/10.1534/genetics.112.147850
  10. Van Tassell, C. P., et al. (2008). SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries. Nature Methods, *5*(3), 247-252. https://doi.org/10.1038/nmeth.1185
  11. Legarra, A., Aguilar, I., & Misztal, I. (2009). A relationship matrix including full pedigree and genomic information. Journal of Dairy Science, *92*(9), 4656-4663. https://doi.org/10.3168/jds.2009-2061
  12. Habier, D., Fernando, R. L., & Dekkers, J. C. M. (2007). The impact of genetic relationship information on genome-assisted breeding values. Genetics, *177*(4), 2389-2397. https://doi.org/10.1534/genetics.107.081190
  13. Daetwyler, H. D., Villanueva, B., & Woolliams, J. A. (2008). Accuracy of predicting the genetic risk of disease using a genome-wide approach. PLoS ONE, *3*(10), e3395. https://doi.org/10.1371/journal.pone.0003395
  14. Christensen, O. F., & Lund, M. S. (2010). Genomic prediction when some animals are not genotyped. Genetics Selection Evolution, *42*(1), Article 2. https://doi.org/10.1186/1297-9686-42-2
  15. Garrick, D. J., Taylor, J. F., & Fernando, R. L. (2009). Deregressing estimated breeding values and weighting information for genomic regression analyses. Genetics Selection Evolution, *41*, Article 55. https://doi.org/10.1186/1297-9686-41-55
  16. Habier, D., Fernando, R. L., Kizilkaya, K., & Garrick, D. J. (2011). Extension of the Bayesian alphabet for genomic selection. BMC Bioinformatics, *12*, Article 186. https://doi.org/10.1186/1471-2105-12-186
  17. Zhang, B., & Fan, T. (2022). Knowledge structure and emerging trends in the application of deep learning in genetics research: A bibliometric analysis (2000–2021). Frontiers in Genetics, *13*, Article 951939. https://doi.org/10.3389/fgene.2022.951939
  18. Magalhães, A. F. B., Schenkel, F. S., Garcia, D. A., Gordo, D. G. M., Tonussi, R. L., Espigolan, R., Silva, R. M. O., Braz, C. U., Fernandes Júnior, G. A., Baldi, F., Carvalheiro, R., Boligon, A. A., de Oliveira, H. N., Chardulo, L. A. L., & de Albuquerque, L. G. (2019). Genomic selection for meat quality traits in Nelore cattle. Meat Science, *148*, 32-37. https://doi.org/10.1016/j.meatsci.2018.09.010
  19. Wallen, S. E., Lillehammer, M., & Meuwissen, T. H. E. (2017). Strategies for implementing genomic selection for feed efficiency in dairy cattle breeding schemes. Journal of Dairy Science, *100*(8), 6327-6336. https://doi.org/10.3168/jds.2016-11458
  20. Çelik, Ş. (2024). Genomic selection in animal breeding: A bibliometric review. Frontiers in Genetics, *15*, Article 1402140. https://doi.org/10.3389/fgene.2024.1402140.

11. Circular Rural Economy and Economic Leakage Reduction: Insights from a Community-Based Resilience Framework from Rural India

Authors: Shahid Iqbal; Nabanita Dey

Keywords: Circular Rural Economy; Rural Resilience; Economic Leakage; Climate Adaptation; Community Enterprises; Local Economic Development; GLIDE Framework; Atmanirbhar Bharat; Sustainable Livelihoods; Rural Transformation.

Page No: 105-122

DIN IJOEAR-JUL-2026-17
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Rural communities across the Global South are increasingly exposed to a convergence of climate variability, geopolitical instability, supply-chain disruptions, and economic volatility. While conventional rural development approaches have primarily focused on agricultural productivity, market integration, and income enhancement, these strategies often overlook structural vulnerabilities associated with economic leakage, external dependency, and limited adaptive capacity. This paper proposes the Circular Rural Economy (CRE) framework as an integrated approach to strengthening rural resilience through local value retention, economic diversification, and collective enterprise development.

Drawing upon resilience theory, circular economy principles, sustainable livelihoods, and local economic development literature, the study introduces the GLIDE (Group Livelihoods for Development) Framework as an operational model for translating Circular Rural Economy principles into practice. Using evidence from GLIDE pilot interventions across multiple Indian states, the paper demonstrates how economic leakage analysis can identify opportunities for local production, wealth retention, and community-led enterprise development. Findings suggest that significant proportions of village expenditures are directed toward externally sourced goods and services that can potentially be produced using local resources, skills, and institutions.

The paper further develops the concept of Economic Leakage Resilience Theory (ELRT), which posits that resilience increases as communities retain a greater share of locally generated wealth and reduce dependence on external supply chains. To support measurement and policy application, a Rural Resilience Index (RRI) is proposed, integrating economic, social, ecological, institutional, and adaptive dimensions of resilience while explicitly incorporating economic leakage as a key indicator.

The study argues that Circular Rural Economy should be viewed not only as a livelihood promotion strategy but also as a climate adaptation, economic localization, and geopolitical resilience framework. By strengthening local production systems, collective enterprises, and community institutions, the GLIDE framework offers a scalable pathway toward resilient, self-reliant, and sustainable rural development. The findings contribute to emerging debates on rural resilience, localization, and adaptive development, while providing practical policy insights aligned with India's Atmanirbhar Bharat agenda and the Sustainable Development Goals.

Keywords: Circular Rural Economy; Rural Resilience; Economic Leakage; Climate Adaptation; Community Enterprises; Local Economic Development; GLIDE Framework; Atmanirbhar Bharat; Sustainable Livelihoods; Rural Transformation.

References
  1. Adger, W. N. (2000). Social and ecological resilience: Are they related? Progress in Human Geography, 24(3), 347–364. https://doi.org/10.1191/030913200701540465
  2. Adger, W. N. (2006). Vulnerability. Global Environmental Change, 16(3), 268–281. https://doi.org/10.1016/j.gloenvcha.2006.02.006
  3. Berkes, F., & Ross, H. (2013). Community resilience: Toward an integrated approach. Society & Natural Resources, 26(1), 5–20. https://doi.org/10.1080/08941920.2012.736605
  4. Carpenter, S. R., Walker, B., Anderies, J. M., & Abel, N. (2001). From metaphor to measurement: Resilience of what to what? Ecosystems, 4(8), 765–781. https://doi.org/10.1007/s10021-001-0045-9
  5. Chambers, R., & Conway, G. (1992). Sustainable rural livelihoods: Practical concepts for the 21st century. IDS Discussion Paper 296. Brighton: Institute of Development Studies.
  6. Ellen MacArthur Foundation. (2015). Towards the circular economy: Economic and business rationale for an accelerated transition. Cowes: Ellen MacArthur Foundation.
  7. Flora, C. B., & Flora, J. L. (2013). Rural communities: Legacy and change (4th ed.). Boulder, CO: Westview Press.
  8. Folke, C. (2006). Resilience: The emergence of a perspective for social–ecological systems analyses. Global Environmental Change, 16(3), 253–267. https://doi.org/10.1016/j.gloenvcha.2006.04.002
  9. Geels, F. W. (2011). The multi-level perspective on sustainability transitions: Responses to seven criticisms. Environmental Innovation and Societal Transitions, 1(1), 24–40. https://doi.org/10.1016/j.eist.2011.02.002
  10. Gunderson, L. H., & Holling, C. S. (Eds.). (2002). Panarchy: Understanding transformations in human and natural systems. Washington, DC: Island Press.
  11. Hickel, J. (2020). Less is more: How degrowth will save the world. London: Penguin Random House.
  12. Hopkins, R. (2008). The transition handbook: From oil dependency to local resilience. Totnes: Green Books.
  13. Intergovernmental Panel on Climate Change (IPCC). (2023). Climate change 2023: Synthesis report. Geneva: IPCC.
  14. Jacobs, J. (1969). The economy of cities. New York: Random House.
  15. Kumar, N., & Kumar, P. (2020). Climate change and Indian agriculture: A review of impacts and adaptation. Current Science, 118(3), 344–350.
  16. Latouche, S. (2009). Farewell to growth. Cambridge: Polity Press.
  17. Meadows, D. H. (2008). Thinking in systems: A primer. White River Junction, VT: Chelsea Green Publishing.
  18. Ostrom, E. (1990). Governing the commons: The evolution of institutions for collective action. Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511807763
  19. Ostrom, E. (2009). A general framework for analyzing sustainability of social-ecological systems. Science, 325(5939), 419–422. https://doi.org/10.1126/science.1172133
  20. Pelling, M. (2011). Adaptation to climate change: From resilience to transformation. London: Routledge.
  21. Pretty, J. (2008). Agricultural sustainability: Concepts, principles and evidence. Philosophical Transactions of the Royal Society B, 363(1491), 447–465. https://doi.org/10.1098/rstb.2007.2163
  22. Putnam, R. D. (2000). Bowling alone: The collapse and revival of American community. New York: Simon & Schuster.
  23. Raworth, K. (2017). Doughnut economics: Seven ways to think like a 21st-century economist. London: Chelsea Green Publishing.
  24. Sachs, J. D. (2015). The age of sustainable development. New York: Columbia University Press.
  25. Scoones, I. (1998). Sustainable rural livelihoods: A framework for analysis. IDS Working Paper 72. Brighton: Institute of Development Studies.
  26. Sen, A. (1999). Development as freedom. Oxford: Oxford University Press.
  27. Shucksmith, M. (2018). Re-imagining the rural: From rural idyll to rural resilience. Journal of Rural Studies, 59, 163–172.
  28. TEEB (The Economics of Ecosystems and Biodiversity). (2010). Mainstreaming the economics of nature: A synthesis of the approach, conclusions and recommendations of TEEB. Geneva: TEEB.
  29. United Nations. (2015). Transforming our world: The 2030 agenda for sustainable development. New York: United Nations.
  30. United Nations Development Programme (UNDP). (2022). Human development report 2021/2022: Uncertain times, unsettled lives. New York: UNDP.
  31. United Nations Office for Disaster Risk Reduction (UNDRR). (2015). Sendai framework for disaster risk reduction 2015–2030. Geneva: UNDRR.
  32. Walker, B., & Salt, D. (2006). Resilience thinking: Sustaining ecosystems and people in a changing world. Washington, DC: Island Press.
  33. World Bank. (2022). Climate and development report: India. Washington, DC: World Bank.

12. Understanding Livestock Technology Adoption and Farmer Decision-Making through Participatory Rural Appraisal: Evidence from an Adopted Village in Maharashtra, India

Authors: V.S. Pande; S.R. Avhad; N.V. Khode; Dr. G. M. Gadegaonkar; G.R.Channa; R.C. Kulkarni; P.V. Jadhav; S.M. Hajare

Keywords: Participatory Rural Appraisal (PRA), Matrix ranking, Consequence diagram, Livestock technology, Technology adoption, Decision-making, Dairy farming, Goat rearing, Backyard poultry, Maharashtra.

Page No: 123-129

DIN IJOEAR-JUL-2026-21
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The present exploratory and participatory study was purposively conducted in Shirol village of Udgir tehsil in Latur district of Maharashtra. The village was adopted by the College of Veterinary and Animal Sciences, Udgir under the Unnat Bharat Abhiyan Scheme. This study explores the dynamics of livestock technology adoption among farmers in Shirol village, utilizing Participatory Rural Appraisal (PRA) techniques. Recognizing the critical role of animal husbandry in rural Indian livelihoods, the research investigates how farmers make decisions regarding modern agricultural practices within a mixed-farming context. Using a whole-village participatory approach, primary data was gathered through PRA tools, specifically technology mapping, matrix ranking, and consequence diagram. The findings revealed that technologies are not simply adopted, but are discontinued, over-adopted, rejected, or reinvented as well based on practical field problems. Matrix ranking demonstrated that farmers prioritize simple, low-cost, and highly effective practices. Furthermore, farmers showed remarkable adaptability by reinventing tools such as creating homemade concentrate feeds and crafting low-cost poultry equipment to minimize daily expenses. While technologies like artificial insemination and vaccination ranked lower in overall farmer preference due to a heavy dependence on external veterinary services and specialized technicians. Ultimately, the integration of these livestock technologies has significantly enhanced animal health, milk yields, and overall socio-economic conditions in the region. However, realizing their full potential is hindered by high initial costs, limited awareness, and irregular technical support. The study concludes that strengthening field-level extension services and improving veterinary accessibility are vital steps toward promoting sustainable livestock development and maximizing technological benefits for rural communities.

Keywords: Participatory Rural Appraisal (PRA), Matrix ranking, Consequence diagram, Livestock technology, Technology adoption, Decision-making, Dairy farming, Goat rearing, Backyard poultry, Maharashtra.

References
  1. Chambers, R. (1994). The origins and practice of participatory rural appraisal. World Development, *22*(7), 953-969.
  2. Chander, M. (2010). Chaff cutters and fodder chaffing: A simple technology worth adoption. Successes and Failures with Animal Nutrition Practices and Technologies in Developing Countries, 133.
  3. Gaurav Jain, A. Singh, J. Singh and R. Jain. (2023). Overview of Basic Livestock Statistics in India. Advances in Veterinary Sciences, *8*, 69-84.
  4. Kumar, D., P. K. Sanyal, N. Wahane, S. Pal, S. Bisen and K. R. Baghel (2014). Evidence of anthelmintic resistance in ruminants of Jashpur district of Chhattisgarh. Indian Journal of Small Ruminants (The), *20*(2), 62-65.
  5. McCracken, J.A., J.N. Pretty and G.R. Conway, G. R. (1988). An introduction to rapid rural appraisal for agricultural development. International Inst. for Environment and Development, London (United Kingdom)
  6. Ministry of Fisheries, Animal Husbandry and Dairying. (2024). Department of Animal Husbandry, Dairying & Fisheries. Basic Animal Husbandry Statistics - 2024. Livestock Census : 2024.
  7. Ministry of Home Affairs. (2011). Census Tables. 15th Census.
  8. Nahar, A., F. A. Mila, R. J. Culas and M. R. Amin. (2022). Assessing the factors and constraints for value chain development of dairy food products in Bangladesh. Heliyon, *8*(10).
  9. Ntume, B., A. S Nalule and Baluka, S. A. (2015). The role of social capital in technology adoption and livestock development. Livestock Research for Rural Development, *27*(9): 181.
  10. Reddy, G.P. and P.S. Janaki Krishna, (2002). Participatory approaches in Agricultural Research: A case study of Andhra Pradesh. Netherland, Biotechnology Programme. Manage. Extn. Res. Rev., 13-27.
  11. Swathi Lekshmi, P. S., R. Venugopalan and K. Padmini, (2009). PRA methods for studying the impact of technology adoption in crop and poultry enterprises in a rural village. Indian Journal of Social Research, *50*(1): 1-14.
  12. Vinod Prakash, A. K. Singh and H. C. Singh. (2013). "PRA tools for real technology dissemination planning: a case study of Bhawanipura village of Etawah district.": 661-664.
  13. Walli T. K. (2010). Urea treatment of straws. Successes and failures with animal nutrition practices and technologies in developing countries. Proceedings of the FAO Electronic Conference, 1-30 September 2010, Rome, Italy. pp-11.

13. Genetic Mechanisms Associated with Physiological, Nutritional Traits and Grain Yield in Pearl Millet (Pennisetum glaucum L.)

Authors: P. Lakhmi Jyothika; N. Sabitha; L Madhavilatha; G Mohan Naidu

Keywords: Pearl millet, Variability, Physiological traits, Nutritional traits, Genetic advance, Heritability, Iron content, Zinc content, Protein content, Biofortification.

Page No: 130-134

DIN IJOEAR-JUL-2026-23
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Abstract

One hundred genotypes of pearl millet including three checks were tested during kharif 2024 in an Alpha Lattice design to understand the genetic mechanisms associated in governing the inheritance of grain yield, nutritional (iron, zinc, and protein content in seeds), and physiological traits (harvest index, relative water content, and SPAD chlorophyll meter reading). Higher variability for iron and zinc; moderate variability for grain yield, harvest index, and protein; and low variability for relative water content and SPAD chlorophyll meter reading were recorded. High heritability and high genetic advance as percent of mean were noted for iron content, zinc content, grain yield per plant, protein content, and harvest index, indicating predominance of additive gene action, and an early and simple selection would be advisable. High heritability coupled with moderate genetic advance over percent mean registered for relative water content indicated the operation of additive gene actions and might consistently manifest in future generations, resulting in increased effectiveness of the breeding program. Moderate heritability along with low genetic advance for SPAD chlorophyll meter reading revealed governance of non-additive genes and can be improved by heterosis breeding as simple selection might not be effective.

Keywords: Pearl millet, Variability, Physiological traits, Nutritional traits, Genetic advance, Heritability, Iron content, Zinc content, Protein content, Biofortification.

References
  1. Andhale, G.R., Shinde, C.S., Bhavsar, V.V., & Barhate, K.K. (2024). Estimation of variability and genetic diversity in different genotypes of pearl millet (Pennisetum glaucum L.). International Journal of Advanced Biochemistry Research, 8(9), 207-213.
  2. Anuradha, N., Satyavathi, C.T., Bharadwaj, C., Sankar, M., Singh, S.P., & Pathy, T.L. (2018). Pearl millet genetic variability for grain yield and micronutrients in the arid zone of India. Journal of Pharmacognosy and Phytochemistry, 7(1), 875-878.
  3. Burton, G.W. (1952). Quantitative inheritance in grasses. Proceedings of Sixth International Grassland Congress, 1, 277-283.
  4. Donald, C.M. (1962). In search of yield. Journal of the Australian Institute of Agricultural Science, 28, 171-178.
  5. Goswami, P.A., Patel, H.S., & Patel, P.R. (2023). Study of genetic variability, heritability and genetic advance for yield and its component traits in pearl millet [Pennisetum glaucum (L.) R. Br.]. Pharma Innovation, 12, 4305-4308.
  6. Jain, S.K., Deewan, D.K.D., Prakash, O., & Sharma, L.D. (2023). Character associations and path coefficient analysis for grain yield and yield contributing traits in pearl millet [Pennisetum glaucum (L.) R. Br.]. Annals of Arid Zone, 62(2), 127-133.
  7. Jaiswal, A., Kumhar, B.L., Kharbas, A.S., Choudhary, K., Gocher, K., Nayak, P.K., & Yadav, T.V. (2025). Analysis of variability parameters in restorer lines of pearl millet [Pennisetum glaucum (L.) R. Br.]. Plant Archives, 25(1), 523-527.
  8. Johnson, H.W., Robinson, H.F., & Comstock, R.E. (1955a). Estimate of genetic and environmental variability in soybean. Agronomy Journal, 47, 314-318.
  9. Johnson, H.W., Robinson, H.F., & Comstock, R.E. (1955b). Genotypic and phenotypic correlation in soybean and their implications in selection. Agronomy Journal, 47, 477-483.
  10. Lush, J.L. (1940). Intra-sire correlation and regression of offspring on dam as a method of estimating heritability of characters. Proceedings of American Society of Animal Production, 33, 292-301.
  11. Naik, B.S.K. (2022). Genetic analysis of grain yield, morpho-physiological and nutritional traits in CGMS based hybrids of pearl millet (Pennisetum glaucum (L.) R. Br.). Ph.D. Thesis. Acharya N. G. Ranga Agricultural University, Guntur.
  12. Panse, V.G., & Sukhatme, P.V. (1961). Statistical methods for agricultural workers (2nd ed.). ICAR, New Delhi.
  13. Priyanka, V., Shanthi, P., Reddy, D.M., & Reddy, B.R. (2019). Genetic variability studies on yield, physiological and nutritional traits in pearl millet [Pennisetum glaucum (L.) R. Br.]. International Journal of Current Microbiology and Applied Sciences, 8(6), 1234-1245.
  14. Singh, J., & Chhabra, A.K. (2018). Genetic variability and character association in advance inbred lines of pearl millet under optimal and drought condition. Ekin Journal of Crop Breeding and Genetics, 4(2), 45-51.
  15. Sivasubramanian, S., & Madhavamenon, P. (1973). Combining ability in rice. Madras Agricultural Journal, 60, 419-421.
  16. Swamynatham, S. (2019). Genetic divergence studies in pearl millet (Pennisetum glaucum (L.) R. Br.). M.Sc. (Ag.) Thesis. Acharya N.G. Ranga Agricultural University, Guntur.
  17. Tandon, H.L.S. (1999). Methods of Analysis of Soils, Plants, Waters and Fertilisers. Fertiliser Development and Consultation Organisation, New Delhi.
  18. Thomas, A.M., Babu, C., & Iyanar, K. (2018). Genetic variability and association studies in pearl millet for green fodder yield and quality traits. Electronic Journal of Plant Breeding, 9(3), 1263-1271.
  19. Varshney, R.K., Shi, C., Thudi, M., Mariac, C., Wallace, J., Qi, P., Zhang, H., Zhao, Y., Wang, X., Rathore, A., & Srivastava, R.K. (2017). Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments. Nature Biotechnology, 35, 969-976.
  20. Weatherley, P.E. (1950). Studies in the water relations of the cotton plant. I. The field measurement of water deficits in leaves. New Phytologist, 49, 81-87.
  21. Yadav, M.K., Sanadya, S.K., Kumar, A., Kumar, A., Kumar, R., & Gupta, P.C. (2022). Genetic variability parameters and inter-relationship among yield and its attributes in pearl millet (Pennisetum glaucum) hybrids. Biological Forum–An International Journal, 14(2), 662-666.

14. The Effect of Giving Dragon Fruit Peel and Papaya Seed Extracts through Drinking Water on the Percentage Commercial Carcass of Native Chicken

Authors: Sitanggang, D. F.; G. A. M. K. Dewi; M. Wirapartha; dan N. P. M. Suartiningsih

Keywords: Native chicken, Dragon fruit peel, Papaya seed, Commercial carcass, Aqueous extract, Feed additive, Natural growth promoter.

Page No: 135-141

DIN IJOEAR-JUL-2026-24
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The prohibition on the use of Antibiotic Growth Promoters (AGP) has encouraged farmers to seek alternative feed additives, such as dragon fruit peel and papaya seeds. Dragon fruit peel contains various antioxidants, including vitamin C, flavonoids, tannins, alkaloids, steroids, and saponins. Papaya seeds contain phytochemical compounds such as saponins, tannins, and flavonoids, which have potential antibacterial properties. This study aimed to determine the effect of administering aqueous extracts of dragon fruit peel, papaya seeds, and their combination through drinking water on the commercial carcass cuts of native chickens. This study used a Completely Randomized Design (CRD) with four treatments and five replications, where each replication consisted of three native chickens. The treatments applied were drinking water without dragon fruit peel and papaya seed aqueous extract (P0), drinking water with 4% dragon fruit peel aqueous extract (P1), drinking water with 4% papaya seed aqueous extract (P2), and drinking water with 2% dragon fruit peel aqueous extract combined with 2% papaya seed aqueous extract (P3). The observed variables included carcass weight and commercial carcass cuts. The results showed that the administration of dragon fruit peel and papaya seed aqueous extracts in drinking water did not have a significant effect (P > 0.05) on carcass weight or commercial carcass cuts of native chickens. It can be concluded that the administration of 4% dragon fruit peel aqueous extract, 4% papaya seed aqueous extract, and the combination of 2% dragon fruit peel aqueous extract and 2% papaya seed aqueous extract was not able to increase the commercial carcass cuts of 10-week-old native chickens.

Keywords: Native chicken, Dragon fruit peel, Papaya seed, Commercial carcass, Aqueous extract, Feed additive, Natural growth promoter.

References
  1. Adnyana, I. G. S., Dewi, G. A. M. K., & Wirapartha, M. (2014). Pengaruh imbangan energi dan protein ransum terhadap karkas ayam kampung betina umur 30 minggu [Skripsi]. Universitas Udayana.
  2. Ariawan, P. T. B., Siti, N. W., & Sukmawati, N. M. S. (2016). Pengaruh pemberian ransum diferentasi dengan probiotik berbasis sari daun pepaya terhadap potongan karkas komersial ayam kampung. Jurnal Peternakan Tropika, *4*(2), 351-365.
  3. Arifin, B., & Ibrahim, S. (2018). Struktur, bioaktivitas dan antioksidan flavonoid. Jurnal Zarah, *6*(1), 21-29.
  4. Corzo, A., & Kidd, M. T. (2004). Dietary amino acid density effects on growth and carcass characteristics of broiler chickens. Poultry Science, *83*(5), 683-689.
  5. Harimurti, S. (1990). Pengaruh level pemberian energi dan protein pakan terhadap persentase berat karkas dan bagian-bagiannya pada ayam potong. Agritech: Jurnal Fakultas Teknologi Pertanian UGM, *10*(1), 11-15.
  6. Khalaji, S., Zaghari, M., Hatami, K., Hedari-Dastjerdi, S., Lotfollahian, H., & Nazarian, H. (2011). Effects of different levels of Thymus vulgaris essential oil and virginiamycin on the performance, immunity, and carcass characteristics of broiler chickens. African Journal of Biotechnology, *10*(61), 13482-13489.
  7. Londok, J. J. M. R., & Rompis, J. E. G. (2018). Pengaruh pembatasan pakan pada periode starter terhadap potongan komersial 2 strain ayam pedaging. In Seminar Nasional VII HITPI (pp. 5-6).
  8. Maheri, N. W. R., Candrawati, D. P. M. A., & Dewi, G. A. M. K. (2022). Penampilan broiler yang diberi jus kulit buah naga melalui air minum. Jurnal Peternakan Tropika, *10*(3), 630-644.
  9. Mahfuds, L. D., Maulana, F. L., Atmpmarsono, U., & Sarjana, T. A. (2009). Karkas dan lemak abdominal ayam broiler yang diberi ampas bir dalam ransum. In Seminar Nasional Kebangkitan Peternakan. Fakultas Peternakan, Universitas Diponegoro Semarang.
  10. Mait, Y. S., Rompis, J. E. G., Tulung, B., Laihad, J., & Londok, J. J. M. R. (2019). Pengaruh pembatasan pakan dan sumber serat kasar berbeda terhadap bobot hidup, bobot karkas dan potongan komersial karkas ayam broiler strain lohman. Zootec, *39*(1), 134-145.
  11. Mustika, C. I., Aldila, I., & Sjofjan, O. W. E. (2014). Pengaruh penambahan tepung kulit buah naga merah (Hylocereus polyrhyzus) dalam pakan terhadap penampilan produksi burung puyuh (Coturnix coturnix japonica) [Thesis]. Universitas Brawijaya.
  12. Noor, M. I., Yufita, E., & Zulfalina. (2016). Identifikasi kandungan ekstrak kulit buah naga merah menggunakan fourier transform infrared (ftir) dan fitokimia. Journal of Aceh Physics Society, *5*(1), 14-16.
  13. North, M. O., & Bell, D. D. (1990). Commercial chicken production manual (4th ed.). The Avi Publishing Company Inc.
  14. Pasang, N. A. (2016). Persentase karkas, bagian-bagian karkas dan lemak abdominal itik lokal (Anas Sp.) yang diberi tepung kunyit (Curcuma Domestica Val.) dalam pakan [Skripsi]. Universitas Hasanuddin.
  15. Peng, W., Talpur, M. Z., Zeng, Y., Zie, P., Lie, J., Wang, S., & Zhang, H. (2022). Influence of fermented feed additive on gut morphology, immune status, and microbiota in broilers. BMC Veterinary Research, *18*(1), 218.
  16. Putra, I. P. A. Y., Dewi, G. A. M. K., & Wirapartha, M. (2021). Pengaruh pemberian jus kulit buah naga terhadap produksi karkas burung puyuh umur 10 minggu. Jurnal Peternakan Tropika, *9*(2), 378-390.
  17. Sinurat, A. P., Purwadaria, T., Togatorop, M. H., & Pasaribu, T. (2003). Pemanfaatan bioaktif tanaman sebagai feed additive pada ternak unggas: Pengaruh pemberian gel lidah buaya atau ekstraknya dalam ransum terhadap penampilan ayam pedaging. Jurnal Ilmu Ternak dan Veteriner, *8*, 139-145.
  18. Soeparno. (2005). Ilmu dan teknologi daging (4th ed.). Gadjah Mada University Press.
  19. Steel, R. G. D., & Torrie, J. H. (1993). Prinsip dan prosedur statistika suatu pendekatan biometrik (M. Syah, Trans.). Gramedia Pustaka Utama.
  20. Sukadana, I., Rahayu, S. S., & Juliarti, N. (2008). Aktivitas antibakteri senyawa golongan triterpenoid dari biji pepaya (Carica papaya L.). Jurnal Kimia, *2*(1), 15-18.
  21. Ulupi, N., Nuraini, H., Parulian, J., & Kusuma, S. Q. (2018). Karakteristik karkas dan non karkas ayam broiler jantan dan betina pada umur pemotongan 30 hari. Jurnal Ilmu Produksi Dan Teknologi Hasil Peternakan, *6*(1), 1-5. https://doi.org/10.29244/jipthp.6.1.1-5
  22. Yanti, E. P. (2017). Efek pemberian ekstrak biji dan ekstrak daun pepaya (Carica papaya linn) terhadap kadar kolesterol total dan trigliserida tikus putih jantan galur wistar (Rattus norvegicus) yang diberi diet tinggi lemak. Hang Tuah Medical Journal, *15*(1), 1-9.

15. Predatory Mites of the Genus Amblyseius (Acari: Phytoseiidae) Associated with Insect Pests in South Gujarat

Authors: Chaudhari Madhavikumari; Abhishek Shukla

Keywords: Diversity, Mites, South Gujarat, Predatory mite, Amblyseius, Phytoseiidae, Biological control, Whitefly, Bemisia tabaci, Taxonomy.

Page No: 142-148

DIN IJOEAR-JUL-2026-25
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An intensive field investigation was conducted during the year 2024-25 and 2025-26 to study the diversity of predatory mites associated with major agricultural and horticultural insect pests across South Gujarat. The predatory mite family Phytoseiidae emerged as the most dominant and ecologically significant group, represented by a high diversity of species belonging to the genus Amblyseius. During the study period, eight species from genus Amblyseius were identified: Amblyseius channabasavannai, A. largoensis, A. vasiformis, A. multidentatus, A. sacchari, A. gossypii, A. herbicolus and A. longispinosus. These predatory mites were predominantly associated with soft-bodied sucking pests, particularly the whiteflies Bemisia tabaci and Aleurolobus barodensis. The study establishes a critical taxonomic and ecological baseline for deploying these native phytoseiid predators within Integrated Pest Management (IPM) frameworks.

Keywords: Diversity, Mites, South Gujarat, Predatory mite, Amblyseius, Phytoseiidae, Biological control, Whitefly, Bemisia tabaci, Taxonomy.

References
  1. Berlese, A. (1914). Acari nuovi. Redia, *10*, 113-150.
  2. Chant, D. A., & McMurtry, J. A. (2007). Illustrated keys and diagnoses for the genera and subgenera of the Phytoseiidae of the world (Acari: Mesostigmata). Indira Publishing House.
  3. El-Badry, E. A. (1967). Three new species of phytoseiid mites preying on the cotton whitefly, Bemisia tabaci in the Sudan (Acarina, Phytoseiidae). The Entomologist, *100*, 106-111.
  4. Krantz, G. W., & Walter, D. E. (2009). A manual of acarology (3rd ed.). Texas Tech University Press.
  5. McMurtry, J. A., De Moraes, G. J., & Sourassou, N. F. (2013). Revision of the lifestyles of phytoseiid mites (Acari: Phytoseiidae) and implications for biological control strategies. Systematic and Applied Acarology, *18*(4), 297-320.
  6. Moraes, G. J., & Mesa, N. C. (1991). Mites of the family Phytoseiidae of Colombia, with descriptions of three new species. International Journal of Acarology, *17*(2), 117-131.
  7. Nomikou, M., Janssen, A., Schraag, R., & Sabelis, M. W. (2001). Phytoseiid predators as potential biological control agents for Bemisia tabaciExperimental and Applied Acarology, *25*, 271-291.

16. Milling and Aromatic Quality Traits of Rice (Oryza sativa L.) Landraces of Nepal

Authors: Prashansa Pandey; Saurabh Joshi Newar; Bishwash Dhungel; Raju Kharel

Keywords: Nepalese Rice landraces, Milling quality, Head rice recovery, Grain aroma, KOH test.

Page No: 149-162

DIN IJOEAR-JUL-2026-26
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Milling and aromatic quality are two of the most economically important post-harvest attributes that determine the market value and consumer acceptance of rice (Oryza sativa L.). This study evaluated six milling quality traits and grain aroma in 30 rice landraces along with 3 released check varieties of Nepal. Milling quality was assessed in a completely randomized design (CRD) with three replications following the IRRI Rice Knowledge Bank protocol, while grain aroma was scored using the standard 1.7% KOH sensory method on a 1-4 intensity scale by a panel of evaluators. Analysis of variance revealed significant genotypic differences for all studied milling quality traits. Head rice recovery, the most economically valuable milling trait, ranged from 50.07% (Ramkumar-3) to 75.05% (NGRC02829), with NGRC02829 emerging as the best milling-quality genotype. The KOH aroma test classified the 33 genotypes into four groups: 8 non-aromatic, 21 slightly aromatic, 3 moderately aromatic, and 1 strongly aromatic (Kalo Nuniya, mean score 3.50 ± 0.84), a genotype independently documented as an aromatic Nepalese landrace. A Kruskal-Wallis test confirmed significant genotypic variation in aroma intensity (H = 66.84, df = 32, p < 0.001), corroborated by a complementary one-way ANOVA (F = 3.10, p < 0.001), although inter-panelist agreement was low (Fleiss' κ = 0.05), reflecting the well-documented subjectivity of sensory-based aroma scoring. Hierarchical cluster analysis based on the combined milling and aroma trait profile grouped the genotypes into four clusters, separating a superior-milling cluster (headed by NGRC02829) from a poor-milling cluster, and showed that the strongly aromatic landrace Kalo Nuniya did not belong to the best-milling group. Overall, the study identifies landraces with superior milling recovery and distinct aroma expression that represent valuable breeding material for improving the grain quality of Nepalese rice.

Keywords: Nepalese Rice landraces, Milling quality, Head rice recovery, Grain aroma, KOH test.

References
  1. Acharya, S., Ghimire, S., Thapa, R., Bhattarai, P., & Chhetri, B. P. (2024). Evaluation of spring season local and improved rice genotypes on growth, yield, and yield attributing characters in Gorkha district, Nepal. J. La Lifesci, *5*(2), 109-125.
  2. Bao, J. (2018). Rice milling quality. In Handbook of rice: Chemistry and technology (Vol. 2, 4th ed., pp. 339-369). Elsevier, in cooperation with AACC International.
  3. Chen, M. H., & Bergman, C. J. (2005). Influence of grain maturity, milling degree, and milling quality on rice bran phytochemical concentrations. Cereal Chemistry, *82*(1), 4-8.
  4. Dhungel, S., & Acharya, P. (2017). Role of rice in food and nutrition security in Nepal. In Rice science and technology in Nepal (pp. 77-85).
  5. Golam, F., Hui Yin, Y., Masitah, A., Afnierna, N., Majid, N. A., Khalid, N., & Osman, M. (2011). Analysis of aroma and yield components of aromatic rice in Malaysian tropical environment. Australian Journal of Crop Science, *5*(11), 1318-1325.
  6. Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research. John Wiley & Sons.
  7. Hegde, S., Yenagi, N. B., & Kasturiba, B. (2013). Indigenous knowledge of the traditional and qualified ayurveda practitioners on the nutritional significance and use of red rice in medications. Indian Journal of Traditional Knowledge, *12*(3), 506-511.
  8. Joshi, B. K. (2008). Diversity based on coefficient of parentage among rice (Oryza sativa L.) cultivars for mid and high hills of Nepal. Journal of Plant Breeding Group, *1*.
  9. Joshi, B. K. (2015). Rice and wheat gene pools in Nepal (1959-2002). National Agriculture Genetic Resources Center.
  10. Nelson, J. C., Jodari, F., Roughton, A. I., McKenzie, K. M., McClung, A. M., Fjellstrom, R. G., & Scheffler, B. E. (2012). QTL mapping for milling quality in elite western US rice germplasm. Crop Science, *52*(1), 242-252.
  11. Pokhrel, A., Dhakal, A., Sharma, S., & Poudel, A. (2020). Evaluation of physicochemical and cooking characteristics of rice (Oryza sativa L.) landraces of Lamjung and Tanahun districts, Nepal. International Journal of Food Science, *2020*, Article 1589150.
  12. Rai, S., Khadka, D. B., & Pokhrel, B. (2023). Characterization, quality assessment and comparison of selected rice landraces (Anadi, Bhotange, and Kalo Nuniya) of Nepal. Himalayan Journal of Science and Technology, *7*(1), 50-64.
  13. Sharma, S., Pokhrel, A., Dhakal, A., & Paudel, A. (2020). Agro-morphological characterization of rice (Oryza sativa L.) landraces of Lamjung and Tanahun district, Nepal. Annals of Plant Sciences, *9*(2), 3731-3741.
  14. Sood, B. C., & Siddiq, E. A. (1978). A rapid technique for scent determination in rice. Indian Journal of Genetics and Plant Breeding, *38*, 268-271.
  15. Subedi, U., Karki, R., Ojha, P., Mishra, A., Shrestha, M. B., & Dongol, D. M. S. (2018). Degree of milling effect on cold water rice quality. Journal of Nepal Agricultural Research Council, *4*, 7-17.
  16. Thapa, M. J., Shrestha, M. B., Karki, R., & Bhattarai, C. M. (2011). Study on quality and milling recovery of different varieties of rice at varying degree of polishing under Khumaltar condition. Agronomy Journal of Nepal, *2*, 88-92.
  17. Thongbam, P. D., Raychaudhuri, M., Ahmed, T., & Velmurugan, A. (2011). Physicochemical and cooking characteristics of some non-basmati rice varieties of Manipur, India. Rice Science, *18*(3), 175-181.
  18. Tiwari, D. N., Pandey, M. P., Manandhar, H. K., Bhusal, T. N., & Beshir, A. (2025). Exploring diversity in aromatic rice landraces for physio-chemical, cooking and milling quality traits in Nepal. Genetic Resources and Crop Evolution, 1-14.

17. Biology of Angoumois Grain Moth, Sitotroga cerealella Olivier on Sorghum

Authors: R. S. Patel; N. K. Kavad; S. A. Patel; M. R. Siddhapara; V. A. Patil

Keywords: Sorghum, Biology, Angoumois grain moth, Sitotroga cerealella, Stored grain pest, Life cycle, Fecundity, Post-harvest loss.

Page No: 163-169

DIN IJOEAR-JUL-2026-27
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The Angoumois grain moth, Sitotroga cerealella Olivier is one of the most serious pests of sorghum at post-harvest. The experiment was carried out at Biocontrol Laboratory, Department of Entomology, N. M. College of Agriculture, Navsari Agricultural University, Navsari during the period from June to November, 2025 to study the biology of the Angoumois grain moth, Sitotroga cerealella Olivier on sorghum. The study revealed that the incubation period, total larval and pupal period, pre-oviposition, oviposition and post-oviposition period of Angoumois grain moth were 4.42 ± 1.03 days, 26.00 ± 1.89 days, 1.95 ± 0.78 days, 3.03 ± 0.77 and 4.28 ± 1.01 days, respectively. The average hatching percentage of egg was 87.57 ± 4.74%. The average number of eggs laid by a female moth was 100.23 ± 35.87 eggs per female. The average longevity of female moth was 9.35 ± 1.03 days while male moth lived for 7.10 ± 0.77 days. The total life cycle of female and male of S. cerealella were 43.17 ± 2.74 days and 38.30 ± 2.41 days, respectively.

Keywords: Sorghum, Biology, Angoumois grain moth, Sitotroga cerealella, Stored grain pest, Life cycle, Fecundity, Post-harvest loss.

References
  1. Ahmad, R., Hassan, S., Ahmad, S., Nighat, S., Devi, Y. K., Javeed, K., Usmani, S., Ansari, M., Erturk, S., Alkan, M. A., & Hussain, B. (2021). Stored grain pests and current advances for their management. Postharvest Technology - Recent Advances, New Perspectives and Applications. Journal of Information and Technology, *100*, 1-37.
  2. Akter, T., Jahan, M., & Bhuiyan, M. S. I. (2013). Biology of the angoumois grain moth, Sitotroga cerealella (Oliver) on stored rice grain in laboratory condition. Journal of the Asiatic Society of Bangladesh Science, *39*(1), 61-67.
  3. Anonymous. (2025). USDA. Global scenario of the sorghumhttps://ipad.fas.usda.gov/cropexplorer/cropview/commodityView.aspx
  4. Basavanjali, S. N., Prabhuraj, A., & Basavegowda, S. (2020). Biology of angoumois grain moth, Sitotroga cerealella (Olivier) (Gelechiidae: Lepidoptera) on paddy. Journal of Entomology and Zoology Studies, *8*(5), 726-729.
  5. Borzoui, E., Naseri, B., & Ganbalani, G. N. (2016). Effects of food quality on biology and physiological traits of Sitotroga cerealella (Lepidoptera: Gelechiidae). Journal of Economic Entomology, *110*(1), 266-273.
  6. Boshra, S. A. (2007). Effect of high-temperature pre-irradiation on reproduction and mating competitiveness of male, Sitotroga cerealella (Olivier) and their F1 progeny. Journal of Stored Products Research, *43*(1), 73-78.
  7. Demissie, G., Rajamani, S., & Ameta, O. P. (2014). Effect of temperature and relative humidity on development and survival of angoumois grain moth, Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae) on stored maize. International Journal of Sciences: Basic and Applied Research, *15*(2), 9-21.
  8. El-Sherif, S. I., Hashem, M. Y., & Ahmed, S. S. (2008). The life history of the angoumois grain moth, Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae) on maize (Zea mays L.) grains. Egyptian Journal of Agricultural Sciences, *59*(4), 333-338.
  9. Hariprasanna, K., & Patil, J. V. (2015). Sorghum: Origin, classification, biology and improvement. In Sorghum molecular breeding (pp. 3-20).
  10. Kumar, R. (2017). Insect pests of stored grain: Biology, behaviour and management strategies. CRC Press Apple Academic Press.
  11. Kumar, S., Kumar, V., Kumar, A., Sati, K., Raj, P., & Thakur, A. (2022). Studies on biology of Sitotroga cerealella Olivier on stored maize. The Pharma Innovation Journal, *11*(9), 1422-1425.
  12. Muthukumar, M., Kumar, D. V. S. R., Madhumathi, T., & Ahamed, M. L. (2017). Biology of the angoumois grain moth, Sitotroga cerealella (Oliver) on rice varieties. Indian Journal of Entomology, *79*(3), 253-256.
  13. Oganja, Y. H., Maheta, H. Y., Kumar, K., & Bharodia, C. R. (2024). Identification of mutation point and trend analysis of area, production and yield of wheat crop in Gujarat, India. Asian Research Journal of Agriculture, *17*(4), 150-156.
  14. Patel, H. (2019). Biology and nonchemical management of angoumois grain moth, Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae) in stored wheat [M.Sc. thesis, Navsari Agricultural University].
  15. Reed, C. R., Ioerger, B. P., Doyungan, S. M., & Trigo-Stockli, D. (2002). Storage of exported US corn and grain sorghum in tropical climates. Applied Engineering in Agriculture, *18*(6), 707.
  16. Saikia, J., Goswami, M. M., & Bhattacharyya, B. (2014). Biology and detection technique of angoumois grain moth, Sitotroga cerealella Olivier (Lepidoptera: Gelechiidae) on stored rice and maize grains. Journal of Entomology and Zoology Studies, *2*(6), 9-11.
  17. Zote, V. (2023). Bionomics and non-chemical management of angoumois grain moth, Sitotroga cerealella (Olivier) (Gelechiidae: Lepidoptera) on paddy [Ph.D. thesis, Navsari Agricultural University].

18. Effect of Entomopathogens on Angoumois Grain Moth and Seed Viability

Authors: R. S. Patel; N. K. Kavad; S. A. Patel; V. A. Patil

Keywords: Beauveria bassiana, Metarhizium anisopliae, Diatomaceous earth, Sitotroga cerealella, Stored grain pest, Sorghum, Biological control, Seed viability, Entomopathogenic fungi.

Page No: 170-179

DIN IJOEAR-JUL-2026-28
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The laboratory experiment on the effect of entomopathogens on the Angoumois grain mothSitotroga cerealella Olivier revealed that the significantly highest percent adult mortality (72.33%) of S. cerealella was recorded in deltamethrin at 0.04 mL/kg after 72 hours of treatment. In case of entomopathogens, the treatment of M. anisopliae 1.15 WP (1.0 × 108 CFU/g) + diatomaceous earth @ 20 + 5 g/kg was found to be the next effective treatment having 65.80% adult mortality. The significantly lowest percent adult mortality (38.33%) of S. cerealella was recorded in Metarhizium anisopliae 1.15 WP (1.0 × 10⁸ CFU/g) at 10 g/kg after 72 hours of treatment. In case of adult emergence, the significantly lowest adult emergence (2.00) was recorded in deltamethrin at 0.04 mL/kg. The significantly highest seed germination (91.33%) was recorded in treatment of B. bassiana 1.15 WP (1.0 × 10⁸ CFU/g) + diatomaceous earth at 20 + 5 g/kg and M. anisopliae 1.15 WP (1.0 × 10⁸ CFU/g) + diatomaceous earth at 20 + 5 g/kg (91.00%). The significantly lowest seed moisture content (8.24%) was recorded in B. bassiana 1.15 WP (1.0 × 10⁸ CFU/g) + diatomaceous earth at 20 + 5 g/kg and it was statistically at par with M. anisopliae 1.15 WP (1.0 × 10⁸ CFU/g) + diatomaceous earth at 20 + 5 g/kg (8.41%).

Keywords: Beauveria bassiana, Metarhizium anisopliae, Diatomaceous earth, Sitotroga cerealella, Stored grain pest, Sorghum, Biological control, Seed viability, Entomopathogenic fungi.

References
  1. Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, *18*(2), 265-267.
  2. Adams, J. M., & Schulten, G. G. M. (1978). Loss caused by insects, mites and micro-organisms in post-harvest grain loss assessment methods. Journal of Stored Products Research, *15*, 15-17.
  3. Anonymous. (2025). Agresco (21st PPSC) report submitted to Navsari Agricultural University, Navsari (pp. 28-43).
  4. Anuradha, & Bera, A. (2025). Seed treatment with entomopathogens in paddy storage. Uttar Pradesh Journal of Zoology, *46*(22), 120-126.
  5. Bartlett, M. S. (1947). The use of transformation. Biometrics, *3*(1), 39-52.
  6. Bhargava, B. S., & Raghupathi, H. B. (1993). Analysis for plant materials for macro and micronutrients. In Methods of analysis of soils, plants, water and fertilizers (pp. 49-82).
  7. Boshra, S. A. (2007). Effect of high-temperature pre-irradiation on reproduction and mating competitiveness of male, Sitotroga cerealella (Olivier) and their F1 progeny. Journal of Stored Products Research, *43*(1), 73-78.
  8. Cox, P. D., & Wilking, D. R. (1996). The potential use of biological control of pests in stored grain. Home Grown Cereals Authority Research Review, *36*, 53.
  9. Ebeling, W. (1971). Sportive dusts for pest control. Annual Review of Entomology, *16*, 123-158.
  10. Flinn, P. W., & Scholler, M. (2012). Biological control: Insect pathogens, parasitoids and predators. Stored Product Protection, *156*, 203-212.
  11. Korunic, Z. (1998). Diatomaceous earths, a group of natural insecticides. Journal of Stored Products Research, *34*, 87-97.
  12. Kumar, R. (2017). Insect pests of stored grain: Biology, behaviour and management strategies. CRC Press Apple Academic Press.
  13. Mewis, I., & Ulrichs, C. (2001). Action of amorphous diatomaceous earth against different stages of the stored product pests Tribolium confusum (Coleoptera: Tenebrionidae), Tenebrio molitor (Coleoptera: Tenebrionidae), Sitophilus granarius (Coleoptera: Curculionidae) and Plodia interpunctella (Lepidoptera: Pyralidae). Journal of Stored Products Research, *37*, 153-164.
  14. Moore, D., Lord, J. C., & Smith, S. M. (2000). Pathogens. In Alternatives to pesticides in stored-product IPM (pp. 193-227). Springer US.
  15. Mostafalou, S., & Abdollahi, M. (2013). Pesticides and human chronic diseases: Evidences, mechanisms and perspectives. Toxicology and Applied Pharmacology, *268*(2), 157-177.
  16. Muthukumar, M., Kumar, D. V. S. R., Madhumathi, T., & Ahamed, L. (2016). Efficacy of diatomaceous earth in combination with certain entomopathogenic fungi against of Sitotroga cerealella (Olivier) and Rhyzopertha dominica (Fab.) in paddy during storage. The Andhra Agricultural Journal, *63*(1), 158-162.
  17. Prakash, A., Rao, J., Gupta, S. P., & Behra, J. (1993). Evaluation of botanical pesticides as grain protectants against rice weevil, S. oryzae Linn. Botanical Pesticides in Integrated Pest Management, *17*(1), 360-365.
  18. Rajasri, M., Rao, P. S., & Kumari, K. V. S. M. (2012). Inert dusts better alternatives for the management of angoumois grain moth, Sitotroga cerealella in stored rice. International Journal of Science and Research, *3*(10), 278-283.
  19. Salem, S. A., Abou-Ela, R. G., Matter, M. M., & El-Kholy, M. Y. (2007). Entomocidal effect of Brassica napus extracts on two stored pests, Sitophilus oryzae (L.) and Rhyzopertha dominica (Fab.) (Coleoptera). Journal of Applied Sciences Research, *3*(4), 317-322.
  20. Theertha, C. P., Siddaraju, R., Parashivamurthy, C., Naik, M., & Krishna, T. V. (2023). Impact of entomopathogens and inert dust seed treatment on insect pests and seed quality of cowpea (Vigna unguiculata L.) during storage. Mysore Journal of Agricultural Sciences, *57*(3), 401-410.
  21. Yadav, A. (2024). Studies on the effect of entomopathogen and inert dust on storage insect pest and seed quality of wheat under ambient condition [wheat (Triticum aestivum L.)] [M.Sc. thesis, Chandra Shekhar Azad University of Agriculture and Technology].

19. Integrated Management of Tetranychus urticae Koch in Okra: Current Status, Adoption Challenges and Future Perspectives

Authors: Sanjeet Kumar Singh; Kaushal Kumar Pandey; Vishal Yadav; Abhay Singh

Keywords: Tetranychus urticae, Okra, Integrated pest management, Agricultural extension, Biological control, Host plant resistance, Sustainable agriculture, Two-spotted spider mite, Abelmoschus esculentus, Acaricide resistance, IPM adoption, Farmer Field School.

Page No: 180-194

DIN IJOEAR-JUL-2026-29
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Abstract

Okra (Abelmoschus esculentus L. Moench) is an important vegetable crop cultivated widely in tropical and subtropical regions, with India contributing the largest share of global production. Among the major arthropod pests affecting okra, the two-spotted spider mite, Tetranychus urticae Koch, causes serious economic losses by reducing plant growth, fruit quality, and marketable yield. Effective management of this pest requires an integrated strategy that combines host plant resistance, biological control, cultural practices, judicious use of acaricides, and timely advisory support to farmers. This review critically examines published research on the biology, host range, damage potential, resistance mechanisms, and management approaches for T. urticae in okra. Particular emphasis is placed on integrated pest management (IPM), the role of agricultural extension services, digital advisory systems, and precision agriculture technologies in promoting sustainable mite management. The review also identifies existing research gaps, including the need for standardized resistance-screening methods, economic threshold levels, resistance monitoring, and climate-resilient management strategies. Strengthening collaboration among researchers, extension agencies, and farming communities will be essential for improving the adoption of scientifically validated practices and ensuring sustainable okra production under changing climatic conditions. The findings presented in this review may assist researchers, extension professionals, and policymakers in designing sustainable mite-management programmes suitable for diverse agro-ecological conditions.

Keywords: Tetranychus urticae, Okra, Integrated pest management, Agricultural extension, Biological control, Host plant resistance, Sustainable agriculture, Two-spotted spider mite, Abelmoschus esculentus, Acaricide resistance, IPM adoption, Farmer Field School.

References
  1. Abou-Zaid, A. M. M., Bakr, E. M., Yassin, S. A., & Hameed, N. A. A. (2012). Abundance of three sap-sucking pests on three eggplant cultivars with utilization of Phytoseiulus persimilis Athias-Henriot against Tetranychus urticae Koch. Acarines, *6*, 49-53.
  2. Ali, W., George, D. R., Shiel, R. S., Sparagano, O. A. E., & Guy, J. H. (2012). Laboratory screening of potential predators of the poultry red mite (Dermanyssus gallinae) and assessment of Hypoaspis miles performance under varying biotic and abiotic conditions. Veterinary Parasitology, *187*, 341-344.
  3. Barnes, C. L., Wickwar, D., Yost, M., Creech, E., & Ramirez, R. A. (2024). The effects of water stress, temperature, and plant traits on the outbreak potential of a specialist and generalist spider mite species (Acari: Tetranychidae). Journal of Applied Entomology, *148*(1), 13-25.
  4. Barman, S., & Ghoshal, S. (2009). Effect of mite feeding in relation to depletion of organic, mineral and inorganic compounds in coconut (Cocos nucifera). Environment and Ecology, *27*(4A), 1746-1748.
  5. Barman, S., & Ghoshal, S. (2011). Biochemical changes in relation to infestation of Tetranychus ludeni Zacher on chilli (Capsicum frutescens). Environment and Ecology, *29*(2A), 881-884.
  6. Bernardi, D., Botton, M., Cunha, U. S., Bernardi, O., Malausa, T., & Garcia, M. S. (2013). Effects of azadirachtin on Tetranychus urticae (Acari: Tetranychidae) and its compatibility with predatory mites (Acari: Phytoseiidae) on strawberry. Pest Management Science, *69*, 75-80.
  7. Deguine, J.-P., et al. (2021). Integrated pest management: Good intentions, hard realities. Agronomy for Sustainable Development, *41*, Article 38.
  8. East, D. A., Edelson, J. V., & Harris, M. K. (1992). Evaluation of screening methods and search for resistance in muskmelon (Cucumis melo L.) to the two-spotted spider mite, Tetranychus urticae Koch. Crop Protection, *11*, 39-44.
  9. El-Adawy, A. M., & El-Esnawy, B. A. (2005). Economic injury level for the two-spotted spider mite, Tetranychus urticae Koch, on cucumber under plastic house conditions. Egyptian Journal of Agricultural Research, *83*(3), 1217-1223.
  10. Food and Agriculture Organization of the United Nations. (n.d.). From farmer field school to community IPM. FAO Regional Office for Asia and the Pacific.
  11. Frontiers in Sustainable Food Systems. (2025). Constraints and opportunities on okra (Abelmoschus esculentus) production in Ethiopia: A review.
  12. Geroh, M. (2007). Ecology and management of Tetranychus urticae Koch on okra (Abelmoschus esculentus L.) [Doctoral dissertation, CCS Haryana Agricultural University].
  13. Ghoshal, S. (2013). Population dynamics and biochemical fluctuations in relation to the infestation of Tetranychus neocaledonicus André on the leaves of Ocimum sanctumInternational Journal of Life Science, Biotechnology and Pharma Research, *2*(3), 225-231.
  14. Guo, Z. X., & Yue, H. X. (2009). Effects of trichomes of plant surface on mites and its enlightenment on biological control of pest mites. Chinese Bulletin of Entomology, *46*(2), 210-215.
  15. Hildebrand, D. F., Rodriguez, J. G., Brown, G. C., & Volden, C. S. (1986). Twospotted spider mite (Acari: Tetranychidae) infestations on soybeans: Effect on composition and growth of susceptible and resistant cultivars. Journal of Economic Entomology, *79*(4), 915-921.
  16. Hoque, M. F., Khalequzzaman, M., & Wahedul Islam. (2010). Population dynamics of Tetranychus urticae Koch and Phytoseiulus persimilis Athias-Henriot on three host plants. Pakistan Entomologist, *32*(1), 6-11.
  17. Insects. (2025). Acaricide resistance monitoring and structural insights for precision Tetranychus urticae management. Insects, *16*(5), Article 440.
  18. Journal of Economic Entomology. (2020). Drones: Innovative technology for use in precision pest management. Journal of Economic Entomology, *113*(1), 1-25.
  19. Kim, G. H., Song, C., Chang, B. Y., Park, N. J., & Cho, K. Y. (1995). Stability of dicofol resistance of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Korean Journal of Applied Entomology, *34*(1), 61-64.
  20. Kumar, D., Raghuraman, M., Singh, R. N., Santeswari, & Singh, J. (2014). Effect of environmental factors on the population of spider mite Tetranychus urticae Koch on okra in Varanasi region. The Ecoscan, *6*(Special Issue), 231-235.
  21. Kumar, S., Prasad, S., & Singh, R. N. (2002). Resurgence of two-spotted mite, Tetranychus urticae Koch (Acarina: Tetranychidae) due to acaricides and botanicals on okra. Annals of Plant Protection Sciences, *10*(2), 239-242.
  22. Manual, S. K., Sah, S. B., & Gupta, S. C. (2007). Field screening of some okra cultivars against red spider mite Tetranychus neocaledonicus André. Journal of Applied Zoological Research, *18*(1), 59-61.
  23. Minhajul-Haque, Tamanna-Islam, Najmoon-Naher, & Haque, M. M. (2011). Seasonal abundance of spider mite Tetranychus urticae Koch on vegetable and ornamental plants in Rajshahi. University Journal of Zoology, *30*, 37-40.
  24. Ministry of Agriculture & Farmers Welfare. (n.d.). About Kisan Call Centre (KCC). mKisan Portal.
  25. Muhammad Amjad, Bashir, M. H., Gogi, M. D., Muhammad Aslam, Khuram Zia, Khan, M. A., & Ali, L. (2012). Evaluation of some acaricides against the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) on cotton under laboratory and field conditions. Pakistan Entomologist, *34*(2), 125-129.
  26. Park, Y. L., & Lee, J. H. (2002). Leaf damage and tissue damage of cucumber caused by the two-spotted spider mite (Acari: Tetranychidae). Journal of Economic Entomology, *95*(5), 952-957.
  27. Press Information Bureau. (n.d.). mKisan Portal. Ministry of Agriculture & Farmers Welfare, Government of India.
  28. Roseleen, S. S. J., & Ramaraju, K. (2011). Effect of okra entries on biology of two-spotted spider mite Tetranychus urticae (Koch). Annals of Plant Protection Sciences, *19*(1), 67-70.
  29. Roseleen, S. S. J., & Ramaraju, K. (2012). Resistance of okra against two-spotted spider mite Tetranychus urticae Koch. Annals of Plant Protection Sciences, *20*(1), 126-129.
  30. Roseleen, S. S. J., Ramaraju, K., Verma, A. K., Bhardwaj, S. P., & Gupta, P. R. (2010). Host-plant resistance in okra against the two-spotted spider mite Tetranychus urticae Koch. Pest Management and Economic Zoology, *18*(1-2), 179-187.
  31. Sharma, S. S., Kalra, V. K., Dhankhar, B. S., & Kaushik, H. D. (2001). Assessment of yield losses caused by mite Tetranychus urticae Koch on different varieties/cultivars of okra. Haryana Journal of Horticultural Sciences, *30*(1-2), 128-130.
  32. Singh, S. K. (2017). Screening of okra varieties against two-spotted spider mite (Tetranychus urticae Koch) and its management [Doctoral dissertation, Banaras Hindu University].
  33. Suri, K. S., & Singh, G. (2009). Chemical induction of resurgence in sucking insect pests of rice. Journal of Insect Science, *22*(3), 213-226.
  34. Urbaneja, A., Pascual-Ruiz, S., & Pina, T. (2008). Efficacy of five selected acaricides against Tetranychus urticae (Acari: Tetranychidae) and their side effects on relevant natural enemies occurring in citrus orchards. Pest Management Science, *64*(8), 834-842.
  35. Van Leeuwen, T., Vontas, J., Tsagkarakou, A., Dermauw, W., & Tirry, L. (2010). Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: A review. Pesticide Biochemistry and Physiology.
  36. Waddington, H., Snilstveit, B., Garcia Hombrados, J., Vojtkova, M., Anderson, J., & White, H. (2012). Farmer field schools for improving farming practices and farmer outcomes in low- and middle-income countries: A systematic review. Campbell Systematic Reviews.
  37. Yanar, D., Kadoglu, I., & Gokce, A. (2011). Acaricidal effects of different plant part extracts on the two-spotted spider mite (Tetranychus urticae Koch). African Journal of Biotechnology, *10*(55), 11745-11750.
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